凉茶药材布渣叶及其混伪品的DNA条形码鉴定

汤欢,师玉华,屠鹏飞,郑希龙,孙伟,叶萌

中国药学杂志 ›› 2015, Vol. 50 ›› Issue (17) : 1479-1484.

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中国药学杂志
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中国药学杂志 ›› 2015, Vol. 50 ›› Issue (17) : 1479-1484. DOI: 10.11669/cpj.2015.17.004
中药质量控制与安全性检测

凉茶药材布渣叶及其混伪品的DNA条形码鉴定

  • 汤欢1,2,师玉华2,屠鹏飞3,郑希龙4,孙伟2*,叶萌1*
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Identification of Herbal Tea Ingredient Microcos paniculata and Its Adulterants Using DNA Barcoding

  • TANG Huan1,2, SHI Yu-hua2, TU Peng-fei3, ZHENG Xi-long4,SUN Wei2*, YE Meng1*
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摘要

目的 探索一种新的鉴定凉茶药材布渣叶及其混伪品海南破布叶和山苦茶的方法,为其市场监管及用药安全提供保障。方法 该研究选用ITS2序列作为DNA条形码鉴定凉茶药材布渣叶及其混伪品,共收集了56份药材及其基原植物样本,通过提取其基因组DNA,PCR扩增ITS2序列并进行双向测序,运用CodonCode Aligner V3.7.1软件对测出的序列进行校对拼接后,采用MEGA6.0软件进行分析,运用此软件中的K2P模型计算布渣叶及其混伪品的种内、种间遗传距离,构建系统进化NJ树进行分析。结果 布渣叶ITS2序列长度为237 bp,种内遗传距离为0~0.036,远小于其与混伪品间的遗传距离0.155~0.404,NJ树结果显示,布渣叶与其混伪品均可准确区分。结论 基于ITS2序列的DNA条形码技术可准确、有效地鉴定凉茶药材布渣叶及其混伪品,为布渣叶药材的鉴定提供了一种新的分子鉴定手段,为其市场监管及用药安全提供了有力保障。

Abstract

OBJECTIVE To explore a new method to identify Microcos paniculata to guarantee its safe use. METHODS ITS2 Sequence was used as a barcode to identify herbal tea ingredient M. paniculata and its adulterants. The genomic DNAs from 56 samples were extracted, and the ITS2 sequences were amplified and bidirectionally sequenced. All the sequences were assembled and obtained using the CodonCode Aligner V3.7.1. The genetic distances were computed by kimura 2-parameter (K2P) model and the neighbor-joining (NJ) phylogenetic trees were constructed using MEGA6.1. RESULTS The length of ITS2 sequence of M. paniculata was 237 bp. The intra-specific genetic distances(0-0.036) were much shorter than the inter-specific genetic distances (0.155-0.404) between M. paniculata and its adulterants. The NJ tree indicated that M.paniculata and its adulterants could be distinguished clearly. CONCLUSION ITS2 Barcode can accurately and effectively distinguish herbal tea ingredient M.paniculata from its adulterants, which provides a new molecular method to identify M.paniculata from its adulterants. It is a great help for the market supervision and medication safety.

关键词

DNA条形码 / ITS2 / 布渣叶 / 混伪品

Key words

DNA barcoding / ITS2 / Microctis Foliumo / adulterant

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汤欢,师玉华,屠鹏飞,郑希龙,孙伟,叶萌. 凉茶药材布渣叶及其混伪品的DNA条形码鉴定[J]. 中国药学杂志, 2015, 50(17): 1479-1484 https://doi.org/10.11669/cpj.2015.17.004
TANG Huan, SHI Yu-hua, TU Peng-fei, ZHENG Xi-long,SUN Wei, YE Meng. Identification of Herbal Tea Ingredient Microcos paniculata and Its Adulterants Using DNA Barcoding[J]. Chinese Pharmaceutical Journal, 2015, 50(17): 1479-1484 https://doi.org/10.11669/cpj.2015.17.004
中图分类号： R282   

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中国中医科学院自主课题(Z2013363-865)
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