Molecular Identification of Hippocampus and Its Adulterants Based on COI Barcode
ZHANG Gai-xia1,2, LIU Jin-xin3, JIA Jing1, SUN Wei4, SUN Zhi-ying2, SHI Lin-chun1*
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China; 2.College of Chinese Medicine, Shandong University of Traditional Chinese Medicine, Ji��nan 250355, China; 3. Hebei Key Laboratory of Study and Exploitation of Chinese Medicine, Chengde Medical College, Chengde 067000, China; 4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Beijing 100700,China
Abstract��OBJECTIVE To establish an accurate identification method of genuine Hippocampus based on COI sequences as DNA barcodes and analyze the relationship between the genuine and adulterants of Hippocampus. METHODS Two hundred and eleven pieces of COI sequences were collected from 21 Hippocampus species in this study. After PCR amplification and sequencing of the DNAs, the DNA barcoding gap and phylogenetic cluster analysis were carried out. RESULTS The COI sequences of the five authentic species all had obvious DNA barcoding gap. For phylogenetic cluster analysis, all of the samples were monophyletic and every species could be discriminated clearly. CONCLUSION COI is an effective DNA barcode for the identification of the genuine Hippocampus and its adulterants, and have important significance for clinical medication safety.
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ZHANG Gai-xia, LIU Jin-xin, JIA Jing, SUN Wei, SUN Zhi-ying, SHI Lin-chun. Molecular Identification of Hippocampus and Its Adulterants Based on COI Barcode. Chinese Pharmaceutical Journal, 2015, 50(17): 1469-1473.
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