Effects of Nine Flavonoids in Buguzhi on the Proliferation, Differentiation of on Postmenopausal Women��s Osteoblasts and Their Structure-Activity Relationship Analysis
GAO Qian-qian1,2, WENG Ze-bin1,2, YAN Cui-ping3, ZHAO Gen-hua1,2, CHEN Zhi-peng1,2, CAI Bao-chang1,2, LI Wei-dong1,2*
1. Nanjing University of Chinese Medicine Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing 210046, China; 2.Pharmacy College of Nanjing University of Chinese Medicine, Nanjing 210046, China; 3. Jiangsu Jumpcan Pharmaceutical Group Co., Ltd, Taizhou 225400, China
Abstract��OBJECTIVE To investigate the effects of nine flavonoids in Buguzhi on the proliferation, differentiation of on Postmenopausal women��s osteoblasts and their structure-activity relationship analysis. METHODS Osteogenic cells were obtained from the femur in postmenopausal women. The effects of flavonoids on the proliferation, differentiation and osteoblasts were determined by MTT method, measuring the activity of alkaline phosphatase. RESULTS Among the nine flavonoids, bavachin exhibited the highest proliferation, differentiation activity. The final result showed that their proliferation, differentiation activity may be related to the parent structure, the position of hydroxyl group and prenyl group. The results demonstrate that flavonone parent, 7-OH, 6-prenyl may improve the proliferation, differentiation effect. CONCLUSION The results demonstrated that the effects of nine flavonoids in Buguzhi on proliferation, differentiation activity had connection with their structures.
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GAO Qian-qian, WENG Ze-bin, YAN Cui-ping, ZHAO Gen-hua, CHEN Zhi-peng, CAI Bao-chang, LI Wei-dong. Effects of Nine Flavonoids in Buguzhi on the Proliferation, Differentiation of on Postmenopausal Women��s Osteoblasts and Their Structure-Activity Relationship Analysis. Chinese Pharmaceutical Journal, 2015, 50(16): 1397-1401.
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2015, Vol. 50 
