Chemical Constituents of Whole Plant of Polygonum chinese L. var hispidum Hook.f.
ZHANG Hong-wu1, WANG Yue1,2, DU Xiao-wei2, DING Gang1, ZOU Zhong-mei1*
1.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193,China; 2.Heilongjiang University of Chinese Medicine, Harbin 150040, China
Abstract��OBJECTIVE To investigate the antioxidant activities and chemical constituents of Polygonum chinese L. var hispidum Hook.f.METHODS The compounds were isolated by chromatographic techniques with silica gel, Sephadex LH-20 and semi-preparative HPLC. Their structures were identified by analysis of physical and spectral data, and confirmed by comparison of their spectral data with the reported values in the literature or those of authentic samples. The antioxidant activities of the extractions of Polygonum chinese L. var hispidum Hook.f. were evaluated by oxygen radical absorption capacity(ORAC) assay. RESULTS Fifteen compounds were isolated and identified as isopsoralen(1), 2,3,4,6,7-pentamethoxy-9,10-dihydrophenanthrene(2), 1,5,7-tri hydroxy-3-methyl-anthraquinone(3), quercetin(4), gallincin(5), gallic acid(6), heneicosanoic acid(7), nonadecanoic acid(8), arachidic acid(9), triacontanoic acid(10), tetracosanoic acid(11), palmitic acid(12), lacceroic acid(13), ��-sitosterol(14), and daucosterol(15), respectively. Ethyl acetate extracts had stronger antioxidant activities. CONCLUSION All compounds are isolated from the species for the first time, and compound 2 is obtained from this genus for the first time
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ZHANG Hong-wu, WANG Yue, DU Xiao-wei, DING Gang, ZOU Zhong-mei. Chemical Constituents of Whole Plant of Polygonum chinese L. var hispidum Hook.f.. Chinese Pharmaceutical Journal, 2015, 50(12): 1012-1016.
WAN D R, CHEN Y J, WEI L, et al. A study on comparative identification of Polygonum chinense L. and Polygonum chinense L. var. hispidum Hook. f.. J South-Central Univ Nat(Nat Sci Ed)(���������ѧѧ����Ȼ��ѧ��),2009,28(4):54-57.
[3]
XIE S Q, WU P, LIN L D, et al. Identification of chemical constituents from Polygonum chinense Linn. J Trop Sub Bot(�ȴ����ȴ�ֲ��ѧ��),2007(5):450-454.
[4]
YE Q M, JIANG R W, HAN F X, et al. Research general situation of Polygonum chinense L. .Strait Pharm J(��Ͽҩѧ), 2013, 25(10):3-7.
[5]
REN H C, WAN D R, GU J, et al. Cjemicalconstituents from Polygonum chinense L. . Nat Prod Res Dev (��Ȼ�����о��뿪��), 2012,24(17): 1387-1389.
[6]
WANG Y G, XIE S W, SU W W.Study on chemical constituents from Polygonum chinense Linn. . J Chin Med Mater(��ҩ��),2005,28(11):35-36.
[7]
QIU R L, LI L, ZHU M H, et al.Study on the constituents of Psoralea. J Chin Med Mater(��ҩ��),2011,34(8):1211-1213.
[8]
LEONG Y W, KANG C C, HARRION L J, et al.Phenanthrenes, dihydrophenanthrenes and bibenzyls from the orchid Bulbophyllum vaginatum. Phtochemistry, 1997,44(1) :157-165.
[9]
HEMLA T A, SURAJ B K, ALATINONE.An anthraquinone from Cassia Alata. Phtochemistry, 1993,32(6):1616-1617. ZHENG G M, WEI X Y, XU L X, et al.Chemical constituents from pericarp of iongan fruits. Chin Tradit Herb Drugs(�в�ҩ),2011,42(8):1485-1489. MA Z K, DAI Y, ZHANG B B, et al. Study on chemical constituents of Euphorbia stracheyi Boiss. . Northwest Pharm J(����ҩѧ��־),2012,27(1):1-4. DUAN X J, MA H W. Chemical constituentsof the active fraction of Lilium lancifolium Thumb. Chin J Exp Tradit Med Form(�й�ʵ�鷽��ѧ��־),2010,(9):56-57. KAN S, CHEN G, HAN C, et al. Chemical constituents from the roots of Xanthium Sibiricum. Nat Prod Res, 2011,25(13):1243-1249. WU D F, FANG Z J. Analysis of the chemical constituents of the root of Solanum torvumswarz.. J Guangdong Coll Pharm(�㶫ҩѧԺѧ��),2008,24(2):139-140. LIANG Q L, DAI C C, WU Q N, et al. Chemical constituentsin roots of Euphorbia pekinensis. Chin Tradit Herb Drug,(�в�ҩ),2008,27(12):1779-1781. LI Z P, GAO S, HAO G S, et al. Studies on chemical constituents from the flower of Albizzia julibrissin Durazz. China J Chin Mater Med(�й���ҩ��־),2000,25(2):39-40. LU C Y, DENG Y, MEI L, et al. Studies on chemical constituents of Gracilaria lemaneiformis. Chin Tradit Herb Drugs(�в�ҩ),2011,30(6):1069-1071. YU J H, YANG J H, PENG F Z, et al. Studies on chemical constituents of Triptergium wilfordii hook. . J Yunnan Norm Unv(����ʦ����ѧѧ����Ȼ��ѧ��),2003,35(4):52-53. PRIOR R L, HOANG H, GU L, et al. Assays for hydrophilic and lipophilic antioxidant capacity(oxygen radical absorbance capacity(ORAC) of plasma and other biological and food samples. J Agric Food Chem,2003,51(11):3273-3279. SU J F, GUO C J, WEI J Y, et al. Comparative study on the antioxidant capacity of quercetin in vivo and in vitro. Chin J Aappl Physial(�й�Ӧ������ѧ��־),2002,18(4):382-386. XIE X Y, LIU H T, ZHANG J, et al. Study on the antioxidative activity of gallic acid in vitro. J Chongqing Med Univ(����ҽ�ƴ�ѧѧ��),2011,36(3):319-322. WANG G Q, ZHENG Y H, QIAN J F. In vitro antioxidant activity of anthraquinones from rhizome of Reynoutria japonica. J Plant Res Environ(ֲ����Դ�뻷��ѧ��), 2011,20(2): 43-48. LUO Z Y, HUANG X, BAO G R. ESR Study on the scavenging effects of the free anthraquinones from Rheum palmatum L. on superoxide anion radical. Strait Pharm J(��Ͽҩѧ),2009,12(1):43-45.
2015, Vol. 50 
