目的 建立HPLC一测多评法测定冬虫夏草中3种核苷类成分尿苷、鸟苷和腺苷的含量。方法 以腺苷为内参物,建立腺苷与尿苷和鸟苷之间的相对校正因子。分别采用一测多评法和外标法对冬虫夏草中的3种核苷类成分进行测定。结果 通过对测定结果进行比较分析,发现一测多评法和外标法所得的结果没有显著性差异。结论 本实验建立的一测多评法准确可靠,可用于冬虫夏草中核苷类成分的质量评价。

OBJECTIVE To establish a quantitative analysis of multi-components by single-marker (QAMS) method for determination of uridine, guanosine and adenosine in Cordyceps sinensis. METHODS Adenosine was used as the marker to calculate the relative correction factors (RCF) for uridine and guanosine by using high performance liquid chromatography(HPLC). Both QAMS method and external standard method (ESM) were performed to determine the three kinds of nucleosides in Cordyceps sinensis. RESULTS There were no significant differences between the two methods. CONCLUSION The developed QAMS method is feasible to evaluate the quality of Cordyceps sinensis.