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�й�ҩѧ��־ 2015, Vol. 50 Issue (7) :629-633    DOI: 10.11669/cpj.2015.07.014
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CHEN Wei-kang1, MA Shuang-cheng2, LUO Yue-hua1, WU Yan-hong1, XU Yan1*
1.Jiangxi Provincial Research Institute for Drug Control, Nanchang 330029, China;
2.National Institutes for Food and Drug Control, Beijing 100050, China

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Abstract�� OBJECTIVE To establish a UHPLC-MS/MS method for simultaneous determination of tangertin, hesperetin, 5-demethylnobiletin, nobiletin, vitexin, and narirutin in Rukuaixiao granules.METHODS The analysis method was performed on a Waters ACQUITY UPLC�k BEH C18 column(2.1 mm��50 mm,1.7 ��m)with gradient elution of acetonitrile(containing 0.1% formic acid)-0.1% formic acid at a flow rate of 0.6 mL��min-1, and the column temperature was set at 50 ��. Electrospray ionization (ESI) source with positive mode and the detector with multiple reaction monitoring (MRM) mode were adopted to determine the analytes by mass spectormeter.RESULTS Satisfactory linearities were obtained for the six constituents in the investigated ranges (r��0.995 8). The lower limit of quantitation (LOQ) was 0.80, 1.10, 1.30, 0.66, 2.00, 7.10 and 0.70 ng��mL-1, respectively. All the recoveries of samples were between 95.78%-101.35% with RSDs less than 2.1%.CONCLUSION The method is simple, rapid, sensitive, and highly reproducible. It can be applied to the quality control of Rukuaixiao granules.
Keywords�� Rukuaixiao grannles,   UHPLC-MS/MS,   tangertin,   hesperetin,   5-demethylnobiletin,   nobiletin,   vitexin,   narirutin     
�ո�����: 2014-10-20;
ͨѶ���� ����,Ů,������ҩʦ �о�����:��ҩ�������� Tel:(0791)88158716 E-mail:jxyjxuyan@sohu.com   
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CHEN Wei-Kang-, MA Shuang-Cheng-, LUO Yue-Hua- etc .Simultaneous Determination of Six Constituents in Rukuaixiao Granules by UHPLC-MS/MS[J]  Chinese Pharmaceutical Journal, 2015,V50(7): 629-633
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