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�й�ҩѧ��־ 2015, Vol. 50 Issue (7) :590-594    DOI: 10.11669/cpj.2015.07.007
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�׻�������Ҵ���ȡ���TGF-��1�յ����˷��ٰ�ϸ��H358��Ƥ���ʻ��ĸ�Ԥ����
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1.�㽭��ҽҩ��ѧ������һҽԺ,���� 310006;
2.�㽭��ҽҩ��ѧ,���� 310053
Lv Xin1,ZHOU Lin-shui1,YANG Li1,ZHANG Hong1,YANG Jun-chao1,WANG Zhen1,LIU Zheng-zhong2
1.The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, China;
2.Zhejiang Chinese Medical University,Hangzhou 310053, China

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ժҪ Ŀ�� ̽�ְ׻�������Ҵ���ȡ��(EEHD)��ת����������-��1(TGF-��1)�����յ����˷��ٰ�ϸ��H358���ʻ����̵ĸ�Ԥ���á����� ��5 ng��mL-1��ת����������-��1������H358ϸ��24 h,�ɹ�������Ƥ����ת��ģ��,�˺�ֱ����͡��С���Ũ�ȵİ׻�������Ҵ���ȡ������48 h��,ͨ��������ӡ�����붨���ۺ�ø����Ӧ������ϸ����Ƥ��־��E-��𤵰��(E-cadherin)������ϸ����־�ﲨ����(vimentin)�ĵ��׺�mRNA�ı���� 5 ng��mL-1��ת����������-��1�ɳɹ�����H358ϸ����Ƥ����ת��ģ��,�ڲ�ͬŨ�Ȱ׻�������Ҵ���ȡ���Ԥ��,��Ũ������E-��𤵰�ױ�����������,�����ױ���½������� �׻�������Ҵ���ȡ���ܲ��ָ�Ԥ������תת����������-��1�յ���H358ϸ����Ƥ����ת�����̡�
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Abstract�� OBJECTIVE To investigate whether the ethanol extract of Oldenlandia diffusa (EEHD) have impact on the TGF-��1-induced epithelial mesenchymal transformation(EMT)of human lung adenocarcinoma cell H358 in vitro.METHODS The cell model to study EMT of H358 cells was established with 5 ng��mL-1 TGF-��1 for 24 h. Then,after being mixed with low, medium and high concentration of EEHD respectively for 48 h, H358 cells were collected for detection of protein and mRNA expression of epithelial marker E-cadherin and mesenchymal cell marker vimentinby Western-Blot and quantitative PCR.RESULTS The concentration of 5 ng��mL-1 TGF-��1 can successfully induce the transformation from epithelialphenotype to mesenchymal phenotype. The concentration of EEHD is positively correlated with increasing expression of E-cadherin,while negatively correlation with the decreasing expression of vimentin.CONCLUSION The EEHD have partially reversal effect of epithelial mesenchymal transformation on lung adenocarcinoma cell induced by TGF-��1.
Keywords�� Hedyotis diffusa ethanol extract,   epithelial mesenchymal transition(EMT),   H358 cell     
�ո�����: 2014-06-11;
��������:�㽭ʡ���������л���������Ŀ(2013KYB187);�㽭ʡ��ҽҩ����ֿ��л���������Ŀ(2013ZQ014)
���߼��: :���,Ů,˶ʿ,����ҽʦ �о�����:�ΰ������� Tel:(0571)86620305 E-mail:LX1206@163.com
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���, ����ˮ, ������ .�׻�������Ҵ���ȡ���TGF-��1�յ����˷��ٰ�ϸ��H358��Ƥ���ʻ��ĸ�Ԥ����[J]  �й�ҩѧ��־, 2015,V50(7): 590-594
��Xin-, ZHOU Lin-Shui-, YANG Li- etc .Intervention Effect of Ethanol Extract of Oldenlandia diffusa on TGF-��1-Induced Epithelial Mesenchymal Transformation of Human Lung Adenocarcinoma Cell H358[J]  Chinese Pharmaceutical Journal, 2015,V50(7): 590-594
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