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�й�ҩѧ��־ 2015, Vol. 50 Issue (5) :394-398    DOI: 10.11669/cpj.2015.05.004
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DUAN Ying-zi, KE Shao-ying
Department of Life Science, Tangshan Normal University, Tangshan 063000, China

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Abstract�� OBJECTIVE To study variation of total flavonoids from nine different Bupleurum chinense introduced of different ground parts of different organs in different growth period, and explore the best harvest period and the optimal varieties. METHODS The extraction conditions were optimized by microwave assisted method,UV-visible spectropholometry was used to determine the contents of total flavonoids. RESULTS The optimum extraction conditions:the concentration of ethanol was 80%, each microwave time 40 s, microwave 10 times; the content of total flavonoids was difference in different growth period, fluctuated trend. The flower was the highest, with an average of 7.59% in the range of 4.65%-16.75%,the content of total flavonoids in nutrition growth phase:leaf>stem, in flowering stage:flower>leaf>stem; in fruiting stage:the content of Gansu Longxi, Liaoning Shenyang, Shandong Heze and Hebei Anguo Bupleurum:fruit >leaf> stem, other Bupleurum:leaf >stem>fruit;The higher the content of total flavonoids was in the flowering and fruiting of nine different Bupleurum chinense introduced, Gansu Longxi Bupleurum (flowering stage) was the highest, up to 27.41%. CONCLUSION It is suggested that harvested in the flowering or fruiting stage,and carried on the comprehensive development and utilization.
Keywords�� Bupleurum chinense,   total flavonoids,   UV-visible spectropholometry,   flower     
�ո�����: 2015-05-14;
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���߼��: :��Ӣ��,Ů,˶ʿ,������ �о�����:ֲ�����������\���о� Tel:(0315)3863212 E-mail:dyz03247@sohu.com
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��Ӣ��, ����Ӣ .��ͬ���ֲ����ͬ�����ڵ��ϲ��ܻ�ͪ�����Ƚ��о�[J]  �й�ҩѧ��־, 2015,V50(5): 394-398
DUAN Ying-Zi, KE Shao-Ying .Comparative Study on the Total Flavonoids Content in Different Species of Bupleurum in Different Growth Period on the Site[J]  Chinese Pharmaceutical Journal, 2015,V50(5): 394-398
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[1] i>Ch. P(2010).Vol�� (�й�ҩ��2010���. һ��).2010:263.[2] XIE D H,CAI B C,AN Y Q, et al. Advances in studies on chemical constituents and pharmacological effect of saikosaponin. J Nanjing Univ Tradit Chin Med (�Ͼ���ҽҩ��ѧѧ��),2007,23(1):63-65.[3] CHEN S F, CHI R A, TIAN J. The optimum condition and kinetics on extraction flavonoids from Bupleurum chinense DC.. J Wuhan Inst Chem Technol (�人����ѧԺѧ��),2006,28(3):10-13,16.[4] WANG H W,LIU Y Q,LU X E. Optimization of extraction technology of flavonoids from Buplerurum scorzonerifolium Wild.. Fine Chem(��ϸ����),2007,24(6):562-564.[5] YE F,YANG G Y,DU S M,et al. Comparative study on the content of total flavonoids in Bupleurum and the aerial parts from northwest hubei. China Pharm (�й�ҩʦ),2013,15(1):52-54.[6] WANG Q,SHI Y Y,XU Z C,et al. Determination of the content of total flavonoids in Herba Bupleuripart on the ground . J Chin Med Mater (��ҩ��),2005,28(6):453-455.[7] TAN L L,HOU X M,HU Z H. Accumulation locations and contents comparison of total saponins and flavonoids in vegetative organs of Bupleurum scorzonerifolium Willd.. Acta Bot Boreali-Occidentalia Sin(����ֲ��ѧ��),2014,34(2):276-281.[8] MEI Z,YANG J,FAN H J,et al.Determination of flavonoinds from the aerial parts of 4 species of Bupleurum plants . Chin J New Drugs(�й���ҩ��־), 2011,20(10):932-935. [9] YUAN W J,ZHANG W R,WU H X,et al. Determination of four flavonoids in different parts of Bupleurum chinense by HPLC. Chin Tradit Pat Med(�г�ҩ), 2013,35(4):797-800. YUE Z J,GUAN C L,WANG H Q,et al.RP-HPLC Determination of four flavonoids in Radix Bupleuri Chinensis . J Yanbei Teac Coll(ɽ����ͬ��ѧѧ��), 2010,26(4):35-38. YANG J,LIU J Q,WANG B C,et al. Optimization of technology by orthogonal test for extracting total flavonoids from Mori Cortex. Liaoning J Tradit Chin Med (������ҽ��־),2014,41(2):319-321. WANG H N,XIE Y Z,FAN F Y,et al.Microwave extraction of total flavonoids in herba Bupleuri by orthogonal test method. Pharm J Chin PLA(��ž�ҩѧѧ��), 2008,24(4):311-313. XIONG H H,CAI G M,HUANG H H,et al. Extraction technique of active constituents from a Tibetan Bupleurum overground. Cent South Pharm(����ҩѧ), 2007(3):214-216. WANG H X, CONG P, LIU H Z, et al. Research advances about the eefense mechanisms for plants to enhanced Ultraviolet-B radiation. Acta Bot Boreali-Occidentalia Sin(����ֲ��ѧ��),2007,27(7):1491-1497. TAN L L,CAI X,HU Z H.Study on the dynamic variation of total flavonoids in different organs of Bupleurum chinense DC . Chin Tradit Herb Drugs(�в�ҩ), 2008,39(2):286-287. YANG G Y,YE F,DU S M,et al. Dynamic change of total flavonoids in roots and aerial parts of Bupleurum Marginatum in various harvest times from northwest hubei. China Pharm(�й�ҩʦ), 2014,16(3):392-394.
[1] ��Խa, ����b*, �ο�b, ������b, ���b, ���b, �ᄃb, �Զ���b.Խ��ԭ������ͨ��������ͨ·�յ�������ϸ�������Ļ����о�[J]. �й�ҩѧ��־, 2015,50(6): 507-511
[2] ����,����*,����ѧ,����,�ڵ�ΰ.�佺�ܻ�ͪ���Է������򲡴�����֬��л��Ӱ�켰����������[J]. �й�ҩѧ��־, 2015,50(3): 217-220
[3] �ϻ�, ���Ǿ�, ����¡, κ��, ��˫��.�ϲ���볣����αƷ�ļ��𷽷��о�[J]. �й�ҩѧ��־, 2015,50(2): 109-114
[4] ֣��,, ����, Ԭ��*, ����,, ����.���������Ծۺ�ø��ʽ��Ӧ�����컨���ٷ��Ӽ����о�[J]. �й�ҩѧ��־, 2015,50(1): 23-28
[5] �����,,�ξ�Ԫ,����,�ܺ�,Ҧ��*.ITS/ITS2�����������ڻ���ҩ�ļ����е�Ӧ��[J]. �й�ҩѧ��־, 2014,49(7): 534-538
[6] �վ�, , ����, �����, ����˫, ������, �����*.�컨ˮ��ȡ��Ļ�ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2014,49(6): 455-458
[7] ������, Ҧ־��, *, ����, �̺�, ��Ӣ, ����, , Ҧ����, .��ЧҺ��ɫ�׷�ͬʱ�ⶨ�׻��������6���ɷֵĺ���[J]. �й�ҩѧ��־, 2014,49(5): 406-411
[8] ����, �Ӿ�, �ⷱ��*, ������, ����, ����.Ұ�ջ��ܻ�ͪ�Ը���ά�������������ü�����̽��[J]. �й�ҩѧ��־, 2014,49(5): 367-370
[9] ��ѩ��, ������*.�������ֲ����ܻ�ͪ�����������Էβ�����ģ����֢�ĸ�Ԥ���ü������о�[J]. �й�ҩѧ��־, 2014,49(4): 294-297
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[11] ���㺣,��־Զ*,������.��ˮ���������Գɷ��о�[J]. �й�ҩѧ��־, 2014,49(23): 2069-2072
[12] ��ݼݼ,ɳ��Ө,��СӢ*.��ЧҺ��ɫ�׷��ⶨ��ͬ���ؿඹ�Ӹ���λ��6�������[J]. �й�ҩѧ��־, 2014,49(23): 2120-2123
[13] ���ŵ�, ��ѩ÷*, ������*, ֣����, ����, ������, ����, �·���, �����.άҩ��������ҩ��������׼�о�[J]. �й�ҩѧ��־, 2014,49(2): 106-112
[14] ���,����,����Ƽ,�ܶ���,������,��ٻ.���ˮ��Һ�в������a��d���ܳ���ת������[J]. �й�ҩѧ��־, 2014,49(19): 1692-1696
[15] ��һ��, ��ѩ��, ������*, ������*.ˮ�Ż���ֲ�ﻯѧ�ɷֺ�ҩ�������о���չ[J]. �й�ҩѧ��־, 2014,49(18): 1577-1582
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