大别山野葛根异黄酮超声辅助提取工艺的响应面优化与还原力测定

黄仁术,, 胡晓梦, 何惠利

中国药学杂志 ›› 2015, Vol. 50 ›› Issue (1) : 51-57.

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中国药学杂志 ›› 2015, Vol. 50 ›› Issue (1) : 51-57. DOI: 10.11669/cpj.2015.01.013
论著

大别山野葛根异黄酮超声辅助提取工艺的响应面优化与还原力测定

  • 黄仁术1,2, 胡晓梦2, 何惠利2
作者信息 +

Optimization of Ultrasonic-Assisted Extraction Techniques of Isoflavones from Pueraria lobata(Wild) Ohwi in Dabie Mountain by Response Surface Methodology and Reducing Power Measurement

  • HUANG Ren-shu1,2, HU Xiao-meng2 , HE Hui-li2
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文章历史 +

摘要

目的 确定大别山野葛根异黄酮的超声辅助最佳提取工艺与还原力。方法 在单因素实验的基础上,进行4因素5水平的Box-Behnken中心组合实验设计,利用响应面分析优化工艺条件,并测定优化工艺条件下葛根异黄酮提取液的还原力。结果 大别山野葛根异黄酮300 W功率的超声辅助提取最佳工艺条件为乙醇75%、液料比35 mL·g-1、处理时间35 min、处理温度50 ℃,此工艺条件下葛根异黄酮得率为24.18%,相对误差仅3.02%;葛根异黄酮还原力的EC50值为0.135 mg·mL-1结论 响应面法适用于对葛根异黄酮超声辅助提取得率进行回归分析和参数优化。

Abstract

OBJECTIVE To optimize the ultrasonic-assisted extraction process of isoflavones from Pueraria lobata (Wild.) Ohwi using response surface methodology and measure the reducing power. METHODS On the basis of single factor experiments, a four factors and five levels Box-Behnken experiment was designed. Then the reducing power of isoflavones was measured. RESULTS The optimum conditions for the ultrasonic-assisted extraction process of isoflavone from Pueraria lobata (Wild.) Ohwi at ultrasonic power of 300 W were as follows: ethanol concentration 75%, liquid-solid ratio 35 mL·g-1, extraction time 35 min, and treatment temperature 50 ℃. Under the optimum conditions, the yield of polysaccharide from Pueraria lobata(Wild.) Ohwi was 24.18%, and the relative error between experimental value and predicted value was only 3.02%; the EC50 of the reducing power of isoflavones reached 0.135 mg·mL-1.CONCLUSION The response surface methodology is suitable for regression analysis and optimization of the ultrasonic-assisted extraction of polysaccharide from Pueraria lobata (Wild.) Ohwi.

关键词

葛根 / 异黄酮 / 响应面 / 超声辅助法 / 工艺 / 还原力

Key words

Pueraria lobata(Wild.) Ohwi / isoflavone / response surface methodology / ultrasonic-assisted / extraction process / reducing power

引用本文

导出引用
黄仁术,, 胡晓梦, 何惠利. 大别山野葛根异黄酮超声辅助提取工艺的响应面优化与还原力测定[J]. 中国药学杂志, 2015, 50(1): 51-57 https://doi.org/10.11669/cpj.2015.01.013
HUANG Ren-shu,, HU Xiao-meng , HE Hui-li. Optimization of Ultrasonic-Assisted Extraction Techniques of Isoflavones from Pueraria lobata(Wild) Ohwi in Dabie Mountain by Response Surface Methodology and Reducing Power Measurement[J]. Chinese Pharmaceutical Journal, 2015, 50(1): 51-57 https://doi.org/10.11669/cpj.2015.01.013
中图分类号: R284.2   

参考文献

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基金

2014年安徽省高校优秀青年人才支持计划;安徽省高等学校省级自然科学研究重点项目(KJ2012A280);国家级大学生创新创业训练计划项目(201310376002)
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