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�й�ҩѧ��־ 2015, Vol. 50 Issue (3) :263-268    DOI: 10.11669/cpj.2015.03.017
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LC-MS/MS�ⶨ����Ѫ����16�ֵ�֭�ἰ�ڸʲݼ����о��е�Ӧ��
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1.���ϴ�ѧ���Ŷ�ҽԺҩѧ��, ���ϴ�ѧ�ٴ�ҩѧ�о���, ��ɳ 410011;
2.������ҽҩ��ѧҩѧԺ, ��ɳ 410208
DENG Yang1,2,ZHANG Bi-kui1,YAN Miao1*,GONG Hui1,LI Huan-de1,FANG Ping-fei1,FAN Xin-rong2, HE Gui-xia2, CAI Hua-lin1
1.Department of Pharmacy, The Second Xiangya Hospital, Central South University, Institute of Clinical Pharmacy, Central South University, Changsha 410011, China;
2. Pharmaceutical College, Hunan University of Chinese Medicine,Changsha 410208, China

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ժҪ Ŀ�� �����ⶨ����Ѫ����16�ֵ�֭��Ũ�ȵ�LC-MS/MS���� ���� ��Ѫ����Ʒ�����״������׺�,����Һ�Ӹ�,�����ิ�ܺ�,��XtimateTM C18������,�Լ״�-����識�����ˮ��ҺΪ����������ݶ�ϴ��,����0.25 mL·min-1,����5 μL������ ��� 16�ֵ�֭����0.005 6~3.800 0 μmol·L-1��,���Թ�ϵ����,��������Ũ��Ϊ0.005 6 μmol·L-1,�͡��С���Ũ�ȵ���ȡ��������70.3%~107.2%֮��,���ڡ��ռ�RSD��С��12.9%�� ���� �÷���׼ȷ�ɿ�,�����ȸ�,������,������Ѫ���е�֭��Ũ�ȵIJⶨ,Ϊ�ٴ�Ѫ����Ʒ�е�֭�Ậ���ⶨ�ṩ�ο���
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Abstract�� OBJECTIVE To establish an LC-MS/MS method for determination of the concentrations of 16 bile acids in rat plasma. METHODS The plasma protein was precipitated with methanol and then the supernatant was dried. Liquid chromatographic separation was performed on a XtimateTM C18 column with gradient elution of mobile phase consisting of methanol-ammonium acetate and formic acid buffer, the flow rate was 0.25 mL·min-1, and the injection volume was 5 μL. RESULTS The calibration curves were linear in the concentration range of 0.005 6-3.800 0 μmol·L-1. The lower limit of quantification was 0.005 6 μmol·L-1. The recoveries of the samples at low, middle and high concentrations were 70.3%-107.2 %. The intra-day and inter-day RSDs were less than 12.9%. CONCLUSION This method is accurate and sensitive for determination of bile acids in rat plasma, which provides reference for the determination of bile acids in clinical samples.
Keywords�� LC-MS/MS,   bile acid,   rat plasma,   liquorice,   detoxification     
�ո�����: 2015-03-09;
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������Ȼ��ѧ����������Ŀ(81202985;81473411);�����У��������ҵ���������ϴ�ѧ�����ʦ������Ŀ(2012QNZT151)

ͨѶ���� ����,��,��ʿ,����ҩʦ �о�����:����ҩ�������ҩ���л����ѧ Tel:(0731)85292099 E-mail:yanmiaocsu@126.com     Email: yanmiaocsu@126.com
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����, , �űϿ��� .LC-MS/MS�ⶨ����Ѫ����16�ֵ�֭�ἰ�ڸʲݼ����о��е�Ӧ��[J]  �й�ҩѧ��־, 2015,V50(3): 263-268
DENG Yang-, , ZHANG Bi-Kui- etc .LC-MS/MS Method for Determination of Sixteen Bile Acids in Rat Plasma and Its Application in Liquorice Detoxification Research[J]  Chinese Pharmaceutical Journal, 2015,V50(3): 263-268
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