Abstract��
OBJECTIVE To develop an HPLC-MS/MS method coupled with EZ:faastTM amino acid analysis kits for the determination of glutamine in rat plasma for the pharmacokinetic study in rats.METHODS The plasma samples were prepared by EZ:faastTM amino acid analysis kits and then analyzed with API 3000 HPLC-MS/MS system. The analytical column was Phenomenex EZ:faast 4 �� AAA-MS(4 micron, 3.00 mm��250 mm) and the column temperature was 20 ��. The mobile phase was composed of 0.2% formic acid aqueous solution(containing 5 mmol��L-1 ammonium acetate)-methanol and eluted gradiently at a flow rate of 0.4 mL��min-1. The detection was performed with multiple reactions monitoring(MRM) using positive electrospray ionization(ESI). RESULTS The calibration curve for glutamine was linear over the concentration range of 3.003-150.2 ��g��mL-1(r=0.996 6). The lower limit of quantification was 3.003 ��g��mL-1. The inter- and intra-day RSDs were less than 15% and the accuracy was within 85%-115%. The extraction recoveries were around 90%, and glutamine was proved to be stable under different circumstances.CONCLUSION This method is specific, sensitive, and accurate, and the sample preparation procedure is simple, rapid, and suitable for the pharmacokinetic study of glutamine in rats.
WANG Ting,
JIANG Xue-Hua,
WANG Ling-*
.HPLC-MS/MS Method for Determination of Glutamine in Rat Plasma and Application to Pharmacokinetic Study[J] Chinese Pharmaceutical Journal, 2015,V50(3): 253-257
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