目的 建立高效毛细管电泳法(HPCE)同时测定乌头类药材中乌头碱、次乌头碱、新乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱和苯甲酰新乌头原碱含量的方法。方法 以40 mmolL-1乙酸钠-60 mmolL-1乙酸铵-80 mmolL-1tris试剂-25%甲醇(pH 8.3)为缓冲溶液,未涂渍标准熔融石英毛细管(75 μm×64.5 cm, 有效长度56 cm)为分离通道,分离电压为16 kV,检测波长为235 nm,毛细管温度为25 ℃,压力进样为5 kPa×6 s。结果 6种生物碱的浓度与峰面积的线性关系良好(r>0.999 7) ;加样回收率为95.25%～103.91%。用此法测定了乌头类药材中6种生物碱的含量,得到满意结果。结论 该方法简单、准确,重复性较好,可用于乌头类药材内在质量的评价和控制。
Abstract
OBJECTIVE To establish a high performance capillary electrophoresis (HPCE) method for simultaneous determination of aconitine, hypaconitine, mesaconitine, benzoylaconine,benzoylhypaconine and benzoylmesaconine in the herbs from Aconitum plants. METHODS The solution of 40 mmolL-1sodium acetate-60 mmolL-1 ammonium acetate-80 mmolL-1 Tris-25% methanol was used as buffer solution (pH 8.3), uncoated fused silica capillary (64.5 cm×75 μm id, 56 cm of effective length) was used for separation, the separation voltage was 16 kV, detection wavelength was set at 235 nm, column temperature was maintained at 25 ℃, and sample was injected at 5 kPa×6 s. RESUTLS Six alkaloids showed good linearity(r>0.999 7) in the ranges of the tested concentrations, and the average recoveries of the method were between 95.25%-103.91%. CONCLUSION The method is simple, accurate and reproducible, and can be used for quality control of the herbs from Aconitum plants.
关键词
高效毛细管电泳法 /
川乌 /
附子 /
草乌 /
生物碱 /
含量测定
{{custom_keyword}} /
Key words
HPCE /
Aconiti Radix /
Aconiti Lateralis Radix Praeparata /
Aconiti Kusnezoffii Radix /
alkaloids /
determination
{{custom_keyword}} /
中图分类号：
R917
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Ch.P( 2010 ) .Vol I. (中国药典2010 年版.一部) [S]. 2010:36,177.[2] KAO Y P, LIU M J, YUAN Q Z. Chemical constituents and pharmacological activities of Aconite[J]. Shanxi J Tradit Chin Med (陕西中医), 2010, 31(12):1658-1660.[3] AI C, ZHU Y Y, ZHAO C Q. Rencent advances on chemical constituents, pharmacological study and the endophytes of the genus Aconitum[J]. Nat Prod Res Dev (天然产物研究与开发), 2012, 24(2):248-259.[4] LIU F, YU X H, LI F, et al. Determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products by HPLC [J]. Chin J Chin Mater Med (中国中药杂志), 2006, 31(14):1160-1162.[5] SUN L, ZHOU H Y, ZHAO R H, et al. Determination of six kinds of monoester-and diester-alkaloids in Radix Aconitii Lateralis Praeparata by HPLC [J]. Chin Ttadit Herb Drugs(中草药), 2009, 40(1):131-134.[6] LIU X X, CHAO R B. Determination of alkaloids in Radix Aconitii Lateralis Praeparata by RP-ion-pair HPLC [J]. Acta Pharm Sin (药学学报), 2006, 41(4):365-369.[7] HUANG Z F, YI J H, TANG X L, et al. Optimization of ester-type alkaloids determination method in Aconites Medicinal Material [J]. Chin J Exp Tradit Med Form (中国实验方剂学杂志), 2013, 19(14):101-104.[8] XU W C, CHU Y J, LIN D, et al. Study on separation performance of capillary column coated physically with modified hyperbranched polycarbosilane [J]. Chin J Anal Chem (分析化学), 2010, 38(8):1167-1171.[9] SHI C Y, SUN G X, SONG W J. Capillary eletrophoresis fingerprints of Radix Scutellariae and determination of baicalin by CZE [J].Chin Tradit Pat Med(中成药), 2008, 30(4):469-472.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
江苏省中药炮制重点实验室开放式课题(ZYPZ007);江苏高校优势学科建设工程资助项目(ysxk-2010)
{{custom_fund}}
PDF(935 KB)
