HUA Jun-lei1,2, CAI Xue-ping1,2, JU Jian-ming1,2*, WU Fan1,LI Zhen-hua1,2, L�� Han3, MA Li3, LI Wei-lin3
1.Jiangsu Academy of Chinese Medicine, Nanjing 210028, China; 2.School of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China; 3. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
Abstract��
OBJECTIVE To establish a method for determination of six triterpene acids in solid dispersion tablets of Eriobotryae Folium total triterpenoid acids by HPLC-ELSD. METHODS The samples were analyzed with Ultimate XB C18 column ( 4.6 mm��250 mm,5 ��m) and eluted with acetonitrile-methanol-0.5% ammonium acetate solution as mobile phases in gradient mode, the flow rate was 1.0 mLmin-1, the column temperature was 35 ��, the drift tube temperature of ELSD was set at 100 ��,and the flow rate of nitrogen was 2.5 Lmin-1. RESUTLS There were good linear relationship between natural logarithm of peak areas and injection quality in the range of 0.583-5.832, 0.366-3.664, 0.335-3.352, 0.533-5.326, 0.257-2.570, and 0.602-6.018 ��g for rosolic acid, tormentic acid, maslinic acid, corosolic acid, oleanolic acid, and ursolic acid, respectively.The average recoveries of these six triterpene acids were 101.83%(RSD 1.37%), 100.63%(RSD 2.77%), 103.42%(RSD 2.03%), 103.59%(RSD 1.63%), 104.07%(RSD 1.42%), and 104.56%(RSD 1.84%), respectively. CONCLUSION The established method is simple, accurate and repeatable with good resolution, which can be used for the quality control of solid dispersion tablets of Eriobotryae Folium total triterpenoid acids.
HUA Jun-Lei-,
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CAI Xue-Ping- etc
.Simultaneous Determination of Six Triterpene Acids in Solid Dispersion Tablets of Eriobotryae Folium Total Triterpenoid Acids by HPLC-ELSD[J] Chinese Pharmaceutical Journal, 2014,V49(17): 1550-1553
2014, Vol. 49 