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�й�ҩѧ��־ 2014, Vol. 49 Issue (18) :1599-1604    DOI: 10.11669/cpj.2014.18.006
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b.ҩѧԺ��Ȼҩ�ﻯѧϵ;
c. ҩѧԺ��ѧ����ѧϵ,���� 100191)
WANG Ying-honga, ZENG Ke-wub, NING Xian-lingc, LIU Jun-yic, MA Zhi-zhonga*
a. Department of Integration of Chinese and Western Medicine, Preclinical Medicine College;
b. Department of Natural Medicines, School of pharmaceutical Sciences;
c. Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China

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Abstract�� OBJECTIVE To investigate the inhibitory effects of caffeic acid phenethyl ester (CAPE) on LPS-induced inflammatory responses in BV-2 microglial cells and its mechanism.METHODS The model was build by BV-2 microglial cells induced by LPS (1 ��gmL-1), which was used to inspect the cell survivability by means of MTT, investigate the effect of CAPE on the release of inflammatory factor NO, IL-1��, IL-6 by ELISA, and the expression of inflammation-related protein (iNOS, COX-2, I��B, P-I��B, NF-��B, P-NF-��B) were analyzed by used of Western Blot. Finally, the regulatory effects of CAPE on inflammatory transcription factor NF-��B activation were inspected. RESULTS CAPE and LPS affected on BV-2 cells, which are collected after being incubated at 37 �� for 24 or 8 h, then test the release of NO, IL-1��, IL-6. As is shown, LPS (1 ��gmL-1) can injury the cells,and increase inflammatory cytokines. CAPE (5, 10, 20 ��molL-1) can decrease these inflammatory cytokines, down-regulate iNOS, COX-2, I��B, P-I��B expression and restrain NF-��B nuclear translocation. CONCLUSION The anti-inflammatory activity of CAPE may be achieved through inhibiting NF-��B signaling pathway.
Keywords�� BV-2 microglia cell,   neuroinflammation,   caffeic acid phenethyl ester,   anti-inflammation,   lipopolysaccharide     
�ո�����: 2014-10-23;
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ͨѶ���� ������,��,������,˶ʿ����ʦ �о�����:����ҽ����Բ� Tel/Fax:(010)82802404 E-mail:zhma@bjmu.edu.cn     Email: zhma@bjmu.edu.cn
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WANG Ying-Hong-a, ZENG Ke-Wu-b, NING Xian-Ling-c etc .Inhibitory Effects of Caffeic Acid Phenethyl Ester on Inflammatory Responses of Microglial Cells(BV-2)Induced by Lipopolysaccharide[J]  Chinese Pharmaceutical Journal, 2014,V49(18): 1599-1604
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[1] HUANG Y, MUCKE L. Alzheimer mechanisms and therapeutic strategies [J]. Cell, 2012, 148(6): 1204-1222.[2] ZHANG W, LEE L, CHU Y Q, et al. Effects of ginsenoside on nerve cells injuried by A��-induced monocyte supernatant [J]. Chin Pharm J (�й�ҩѧ��־), 2007, 42(21): 1626-1628.[3] XUE Y, LEE H F, XU L Y. Microglia: In inflammation-mediated neurodegenerative diseases[J]. Chin Bull Life Sci (������ѧ), 2007, 19(1): 43-46.[4] TOMOHARU T, SHINICHI K, TOMONORI M, et al.General anesthetics inhibit LPS-induced IL-1�� expression in glial cells [J]. Plos One, 2013, 8(12): 1-13.[5] ZHAO W X, WANG L, YANG J L, et al. Caffeic acid phenethyl ester attenuates pro-inflammatory and fibrogenic phenotypes of LPS-stimulated hepatic stellate cells through the inhibition of NF-kappaB signaling [J]. Int J Mol Med, 2014, 33(3): 687-694.[6] KOKSEL O, OZDULGER A, TAMER L, et al. Effects of caffeic acid phenethyl ester on lipopolysaccharide-induced lung injury in rats [J]. Pulm Pharmacol Ther, 2006, 19(2): 90-95.[7] LI Y, DU X F, DU J L. Physiological properties and functions of microglia [J]. Acta Physiol Sin(����ѧ��), 2013, 65(5): 471-482.[8] PERRY V H, NICOLL J A, HOLMES C. Microglia in neurodegenerative disease [J]. Nat Rev Neurol, 2010, 6(4): 193-201.[9] KRAUSE D L, MULLER N. Neuroinflammation, microglia and implications for anti-inflammatory treatment in Alzheimer's disease [J]. Int J Alzheimers Dis, 2010,2010. pii: 732806. doi: 10.4061/2010/732806.[10] BANNO M, MIZUNO T, KATO H, et al. The radical scavenger edaravone prevents oxidative neurotoxicity induced by peroxynitrite and activated microglia [J]. Neuropharmacology, 2005, 48(2): 283-290.[11] YU S C, LI X Y. Effect of ginsenoside on IL-1 beta and IL-6 mRNA expression in hippocampal neurons in chronic inflammation model of aged rats [J]. Acta Pharmacol Sin(�й�ҩ��ѧ��), 2000, 21(10): 915-918.[12] KARIN M, YAMAMOTO Y, WANG Q M. The IKK NF-kappa B system: A treasure trove for drug development [J]. Nat Rev Drug Discov, 2004, 3(1): 17-26.
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[2] ��ѧ��, ���ӻ�, ����, ���, ȫ����*.�����ز�ͬ�ܼ���ȡ��Զ������������յ���С���Ը����˵ı�������[J]. �й�ҩѧ��־, 2014,49(6): 469-472
[3] �̺���, �����*.�������࿹��ҩ����Ѫ�ܼ��������еĺ���ʹ��[J]. �й�ҩѧ��־, 2014,49(6): 512-516
[4] ��ѩ��, ������*.�������ֲ����ܻ�ͪ�����������Էβ�����ģ����֢�ĸ�Ԥ���ü������о�[J]. �й�ҩѧ��־, 2014,49(4): 294-297
[5] ������.����ض������Ҵ��ж�������ռ����ı������û����о�[J]. �й�ҩѧ��־, 2014,49(18): 1610-1614
[6] Ф����������ƽ���������������¿�������ɽ*.��������ص�ҩ�����ü�Ӧ���о���չ[J]. �й�ҩѧ��־, 2014,49(13): 1093-1098
[7] �������ף����*�������������������ѧ��.�����ἰ�������￧���ᱽ����ҩ�������о���չ[J]. �й�ҩѧ��־, 2013,48(8): 577-581
[8] ��ε��,�����,����,������*.��׼�������͵Ŀ��������о�[J]. �й�ҩѧ��־, 2013,48(5): 349-353
[9] ������, ����*.��������࿹����ʹֹ�������о�[J]. �й�ҩѧ��־, 2013,48(4): 266-268
[10] ����ӱ���κ��񣬴޿�.3-����-6-�����������ͪ�ĺϳɼ�������ʹ�����о�[J]. �й�ҩѧ��־, 2013,48(3): 224-227
[11] ���޻�,�,������,��Ρ��,�º���,����.ά�������Ŀ�����ʹ�����о�[J]. �й�ҩѧ��־, 2013,48(20): 1736-1739
[12] �»���������*���Գ��������֣�������.�����ݿ��׻��Բ�λ��Ч��ϵ����ЧҺ��ɫ��-Ч��ϵ�о�[J]. �й�ҩѧ��־, 2013,48(14): 1190-1193
[13] ����,����,�*.Ӧ��3T3ϸ���ⶾ��ʵ�����ۼ��ַ����忹��ҩ�Ĺⶾ��[J]. �й�ҩѧ��־, 2013,48(11): 912-915
[14] ��⣬������*���Źڻ�*.��޲�ҩ�����ü��ٴ�Ӧ���о���չ[J]. �й�ҩѧ��־, 2013,48(11): 949-952
[15] ��С��.������ø-2���������Ƽ�������������[J]. �й�ҩѧ��־, 2012,47(5): 399-
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