Abstract��
OBJECTIVE To discuss the availability of evaluation on pharmacokinetics for the system of complicated components by studying the in vivo blood dynamic change of Beagle dogs from Yangxue injection in interval time. METHODS Yangxue injection were administered to dogs by intravenous infusion, plasma samples were collected at different times and treated with methanol to precipitate protein.Area under the absorbance-wavelength curve(AUAWC) and total concentration of sodium ferulate, tetramethylpyrazine hydrochloride and tanshinol sodium in plasma were obtained after a full ultraviolet-visible spectrum scan to samples from 200 to 350 nm wavelength. Meanwhile, individual concentrations of sodium ferulate, tetramethylpyrazine hydrochloride and tanshinol sodium in plasma were determined by HPLC method. The pharmacokinetic parameters by the method of AUAWC and HPLC were obtained after processed with 3P97 procedure from Chinese Pharmacology Institute. RESULTS To Yangxue injection with multicomponent, the former appeared to have a two-compartment model in the Beagle dogs, with pharmacokinetics�� parameters c0 84.94 ��g��mL-1, elimination half-life (t1/2��) 46.94 min and area under concentration versus time (AUC) 3 227.77 min����g��mL-1. The results of the latter by HPLC demonstrated that sodium ferulate and tetramethylpyrazine hydrochloride appeared to have a one-compartment model in the Beagle dogs, c0 were 37.02 and 0.54 ��g��mL-1,t1/2 were 26.24 and 32.35 min,AUC were 1 406.75 and 20.50 ��g��min��mL-1. Tanshinol sodium appeared to have a two-compartment model in the Beagle dogs, with pharmacokinetics�� parameters c0 48.48 ��g��mL-1, elimination half-life (t1/2��) 59.21 min and AUC 1 842.06 ��g��min��mL-1.The results indicated that the total components concentration in plasma and AUC got through the way of AUAWC were greater than that of a single component by HPLC. CONCLUSION The pharmacokinetic characteristics of Yangxue injection in Beagle dogs is shown by the method of AUAWC, which will bring a feasible idea and pathway to pharmacokinetics�� studies of chemicals compound, traditional Chinese medicine with multicomponents and complicated compound of traditional Chinese medicine under the combination between the method of AUAWC and HPLC.
LI Xiao-Dong,
ZHANG Li-Hong,
XIAO Xiao-Jin
.Pharmacokinetics of Yangxue Injection in Beagle Dogs by the Method of Area Under the Absorbance-Wavelength Curve Combined with High Performance Liquid Chromatography[J] Chinese Pharmaceutical Journal, 2014,V49(16): 1442-1447
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2014, Vol. 49 
