Abstract��
OBJECTIVE To model and optimize the reaction conditions of enzymatic esterification of cholesterol with diethenyl decanedioate in isooctane by response surface metodology(RSM). METHODS Central composite design(CCD) was used to design the experiments. Candida rugosa lipase was the biocatalyst for the reaction. The reaction factors investigated were reaction time(t= 4-15 h), enzyme amount(En=2-10 mg) and substrate molar ratio(Sr= 5-10, diethenyl decanedioate-cholesterol). The degree of esterification(DE) was selected as response value. RESULTS All factors investigated positively affected DE. The optimal reaction conditions were: t= 11.47 h, Sr=8.93, En=7.70 mg, and the DE was 90.56% under these conditions. The actual value was in reasonable agreement with the predicted value of 92.80%. CONCLUSION To optimize the enzymatic catalysis synthesis process of cholesteryl vinyl hemi-sebacate by CCD-RSM is effective and feasible.
LUO Li-Hua,
CHENG Yi-*,
NIE Hua etc
.Modeling and Optimization of Lipase-Catalyzed Synthesis of Cholesteryol Vinyl Hemi-Sebacate by Response Surface Methodology[J] Chinese Pharmaceutical Journal, 2014,V49(16): 1426-1431
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2014, Vol. 49 
