Abstract��
OBJECTIVE To prepare nonionic surfactant niosomes by multiple emulsion freeze-drying method and study their encapsulation effects for the water-soluble drug, methotrexate. METHODS Using nonionic surfactants Span60, cholesterol, or PEG 6000 as capsule material, methotrexate niosomes were prepared by the method of multiple emulsion lyophilization. The preparation process was optimized, and the niosomes�� physicochemical properties, entrapment efficiency, stability and in vitro release were investigated. RESULTS The prepared niosomes had uniform particle size distribution and relatively high entrapment efficiency, and the maximum encapsulation efficiency of methotrexate reached 83.71%. The lyophilized product of niosomes had good stability and solubility. The in vitro release experiment showed that the free drug released completely after 5 h, while the cumulative release rate of methotrexate in the niosomes reached only 50%, which indicated the sustained release effect of niosomes. CONCLUSION Multiple emulsion lyophilization is an effective method for the preparation of nonionic surfactant niosomes, which provides a new choice for the development of a novel drug delivery system.
DING Lin-,
WANG Hui-Yun-*,
XIN Shan-Shan- etc
.Preparation of Methotrexate Niosomes Using Freeze-Drying of Double Emulsions Method[J] Chinese Pharmaceutical Journal, 2014,V49(15): 1327-1332
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