LI Qun1, WU Sai-wei1, LIANG Wen-quan2, SUN Xiao-yi3*
1.The Fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu 322000, China; 2.College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China; 3.School of Medicine, Zhejiang University City College, Hangzhou 310015, China
Abstract��
OBJECTIVE To investigate the cellular delivery of calcein-loaded liposomes and the endocytosis mechanism. METHODS Liposomes were prepared by film dispersion method. The in vitro release profiles of calcein solution and calcein loaded liposomes were compared via dialysis method, and the effects of liposome encapsulation on the cellular accumulation, intracellular distribution and uptake mechanism of calcein were investigated on A549 cells. RESULTS The in vitro release test showed sustained release of calcein from liposomes, which lasted for 1 week. Liposomes significantly increased the amount of total and intracellular calcein (P<0.01), and calcein distributed mainly in the lysosomes and outside of the nucleus. The mechanism study showed that liposomes transferred calcein into cells mainly by macropinocytosis and clathrin-dependent endocytic pathway. CONCLUSION Liposome encapsulation can increase the amount of intracellular calcein by changing the endocytic pathway of calcein. Liposome encapsulation is a potential and feasible way for enhancing cellular delivery.
LI Qun-,
WU Sai-Wei-,
LIANG Wen-Quan- etc
.Cellular Delivery of Liposomal Calcein and Its Mechanism Study[J] Chinese Pharmaceutical Journal, 2014,V49(15): 1321-1326
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