Abstract��
OBJECTIVE To establish a high performance capillary electrophoresis(HPCE)method for simultaneous determination of seven nucleosides, including epigoitrin, uracil, thymine, adenosine, hypoxanthine, guanosine and uridine in Isatidis Radix. METHODS The chromatographic separation was performed on an uncoated fused silica capillary(75 ��m��64.5 cm, 56 cm of effective length)with separation voltage of 20 kV. 60 mmol��L-1 Na2B4O7-15 mmol��L-1 ��-CD was selected as the running buffer solution(pH 9.6). The detection wavelength was set at 254 nm. The sample was injected at 5 kPa��5 s and column temperature was maintained at 25 ��. RESULTS The calibration curves of the seven nucleosides showed good linearity (r��0.999 4). The average recoveries of the method were between 97.15%-100.40%. The contents of the seven nucleosides in Isatidis Radix samples of different batches were different. CONCLUSION The established method is simple, accurate and can be used for the quality control of Isatidis Radix.
XIAO Ping,
CHEN Jian-Wei,
LI Xiang-*
.Simultaneous Determination of Seven Nucleosides in Isatidis Radix by HPCE[J] Chinese Pharmaceutical Journal, 2014,V49(7): 604-608
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