Abstract��
OBJECTIVE To optimize the ultrahigh pressure extraction(UPE)process of effective constituents from Jinqiao Reduqing granules by Box-Behnken design-response surface methodology. METHODS On the basis of single factor screening, a three-factor and three-level Box-Behnken experimental design was employed, with solvent ratio(X1), extraction pressure(X2)and extraction time(X3)as independent variables. Dependent variables were transformed into desirabilities mathematically by Hassan��s method. Data of overall desirabilities were fitted to a second order polynomial equation, through which three dimensional response surface graphs were produced. Optimum experimental conditions were selected from the stationary point of the response surfaces. RESULTS Regression coefficient of binomial fitting complex model was as high as 0.987 8. The optimum conditions of UPE were as follows:the materials were extracted for 38 s with 24 times amount of water, the extraction pressure was 112.1 MPa, and the extraction was performed for one time. The bias between observed and predicted values was 5.27%. CONCLUSION As a novel extraction technology for Chinese herbal medicine, the UPE procedure has higher extraction yield, lower extracting temperature, shorter extracting time and less power consumption.The UPE has provided a brand new method for extraction of chlorogenic acid and forsythiaside from Jinqiao Reduqing granules.
YE Chen-Li,
HE Shuai,
KANG Ying-Bo etc
.Optimization of Ultrahigh Pressure Extraction Process of Chlorogenic Acid and Forsythiaside in Jinqiao Reduqing Granules by Box-Behnken Design-Response Surface Methodology[J] Chinese Pharmaceutical Journal, 2014,V49(7): 543-549
��
[1]
CHLOPCIKOVA S, PSOTOVA J, MIKETOVA P, et al. Chemoprotective effect of plant phenolics against anthracycline-induced toxicity on rat cardiomyocytes. Part I. Silymarin and its flavonolignans. Phytother Res, 2004, 18(2):107-110. [2] CHIANG L C, CHIANG W, CHANG M Y. Antiviral activity of Plantago major extracts and related compounds in vitro. Antiviral Res, 2002, 55(1):53-62. [3] KASAI H, FUKADA S, YAMAIZUMI Z, et al. Action of chlorogenic acid in vegetables and fruits as an inhibitor of 8-hydroxydeoxyguanosine formation in vitro and in a rat carcinogenesis model. Food Chem Toxicol, 2000, 38(5):467-471. [4] SHIMODA H, SEKI E, AITANI M. Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice. BMC Complement Altern Med, 2006, 17(6):9. [5] LIU Y, GUO M Y, BAI G B. Advancement of chlorogenic acid. J Chin Med Mater(��ҩ��), 2012, 35(7):1180-1185. [6] FENG S Y, LI X R, SUN J N. The study of forsythoside in anti-infection and abatement of fever. Prog Modern Biomed(�ִ�����ҽѧ��չ), 2006, 6(10):73-75. [7] ZHANG L W, ZHAO C G, YANG P. Study on the antioxidant activity and structure-activity relationship of forsythiaside. Chin Pharm J(�й�ҩѧ��־), 2003, 38(5):334-336. [8] HU J K, XU K J, WANG Y H, et al. Study on the in vitro antiviral effect of forsythiaside. Chin J Tradit Med Sci Tech(�й���ҽҩ�Ƽ�), 2001, 8(2):89. [9] ZHANG L W, YANG P. Study on the stability of forsythiaside. Chin Tradit Pat Med(�г�ҩ), 2003, 25(5):353-356. [10] LIAO G P, HE S, ZHANG Z Y. Optimization of ultrahigh pressure extraction for polydatin and resveratrol from polygonum cuspidatum by using uniform design. China J Chin Mater Med(�й���ҩ��־), 2010, 35(24):3282-3286. [11] LIAO G P, LIU J H, HE S, et al. Optimization for ultrahigh pressure extraction of berberine from cortex phellodendri by central composite design-response surface methodology. J Med Plants Res, 2012, 6(23):3963-3970. [12] GUO X F, HAN D M, XI H P, et al. Extraction of pectin from navel orange peel assisted by ultra-high pressure, microwave or traditional heating:A comparison. Carbohydr Polym, 2012, 88(2):441-448. [13] WU W, CUI G H, LU B. Optimization of multiple variables:application of central composite design and overall desirability. Chin Pharm J(�й�ҩѧ��־), 2000, 35(8):530-533. WU W, CUI G H. Central composite design-response surface methodology and its application in pharmacy. Foreign Med Sci (Sect Pharm)(����ҽѧ��ҩѧ�ֲ�), 2000, 27(5):292-298. [15] YU X L, GUAN Y, YU J P. Optimization of extraction process for Weining capsules by orthogonal test. Tradit Chin Drug Res Pharmacol(��ҩ��ҩ���ٴ�ҩ��), 2006, 17(5):366-367. [16] WANG W J, JIAO X D, WANG X G. Studies on manufacture of Weishu Capsules by orthogonal experiments. Lishizhen Med Mater Med Res(ʱ���ҽ��ҩ), 2005, 16(7):629-630. [17] SHEN Q, ZHOU X, SHI X F, et al. Optimization of extracting techniques of��Zhichuan Capsule��. Shanghai J Tradit Chin Med(�Ϻ���ҽҩ��־), 2007, 41(9):81-83. [18] LIU M Y, WANG B C. Advances in new technologies applying to Chinese materia medica extraction. Chin Tradit Herbal Drugs(�в�ҩ), 2010, 41(2):169-175. [19] XI J, SHEN D J, ZHAO S, et al. Characterization of polyphenols from green tea leaves using a high hydrostatic pressure extraction. Int J Pharm, 2009, 382(1-2):139-143. [20] ZHANG S Q, ZHU J J, WANG C Z. Novel high pressure extraction technology. Int J Pharm, 2004, 278(2):471-474. [21] ZHANG S Q, BI H M, LIU C J. Extraction of bio-active components from Rhodiola sachalinensis under ultrahigh hydrostatic pressure. Sep Purif Technol, 2007, 57(2):277-282. [22] CHEN R Z, ZHANG S Q, LIU Z Q. Application of ultrahigh pressure technology in extracting active component from Chinese materia medica. Chin Tradit Herb Drugs(�в�ҩ), 2007, 38(12):1905-1908. [23] ZHANG S Q, CHEN R Z, WU H, et al. Ginsenoside extraction from Panax quinquefolium L. (American ginseng)root by using ultrahigh pressure. J Pharm Biomed Anal, 2006, 41(1):57-61.
2014, Vol. 49 