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�й�ҩѧ��־ 2014, Vol. 49 Issue (9) :726-730    DOI: 10.11669/cpj.2014.09.005
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MEI Xiao-yun, WU Hao-xin, ZHOU Lan*
Basical Medical College, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China

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ժҪ Ŀ�� �о�����������(BYHWD)�Դ�����ǰ��ʧ�񾭼�ή����Ӱ��,��̽������ơ����� ������������������ģ��,������������Ϊ��������,ģ����,�����������ߡ��С��ͼ�����,���ܰ���,��6��ÿ��10ֻ������ÿ�չ�θ,��ҩ18 d��,ʯ��������Ƭ,��̬ѧ����,������ǰ��ʪ�ر�,����ʵʱӫ�ⶨ���ۺ�ø����Ӧ���Angptl4��PI3K����ı���� ��ʪ�ر�:��ģ�������,�����������ߡ��м�������������(P<0.05��P<0.01)�����˶��հ�:��������Ϊ�غ�ɫ,��״Ϊ��Բ�λ�Բ��,��ɫ����,�����������߼���������ܰ����Ե�ϴֲ�;�����������С��ͼ�������ģ���������𽥼���,����̬������,��Ե���⻬;��ɫҲ�𽥱�dz����Angptl4��PI3K mRNA;�����������ߡ��м�������ģ������Ⱦ���ͳ��ѧ���� (P<0.01��P<0.05)������ �����������������ӻ���ǰ��ʪ�رȽ������˶��հ���˱�,�Ӷ���Ч��ֹʧ�񾭼�ή���ķ���,����ƿ�����ͨ������Angptl4��PI3K�������ʵ�ֵġ�
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Abstract�� OBJECTIVE To explore the influence of buyang huanwu decoction(BYHWD)on the denervated tibial muscle atrophy of rats and to study the mechanisms of BYHWD defering denervated muscle atrophy of rats. METHODS Sprague-Dawley rats were subjected to common peroneal nerve crush model, then they were randomly divided into sham operation group, model group, BYHWD high-dose, medium-dose, low-dose groups, mecobalamin group (positive control). Each group has ten rats. The gavage lasted for 18 d. After that, performed slice, staining and analysis morphology were performed.The wet weight ratio of tibial muscle was measured. At the same time, qRT-PCR and western-blot detect the differential expression of Angptl4 and PI3K in the high-dose group of BYHWD and model group. RESULTS ��Wet weight ratio:BYHWD high and medium-group increased significantly compared with model group(P��0.01 or P<0.05).�� The motor end-plate:the sham operation group is sepia, oval or round; the BYHWD high-dose group and mecobalamin group has little chage; the BYHWD medium-dose group, low-dose group and the model group has lessen, shape is not rule gradually, the edge is rough, coloring is slight gradually. ��the expression of Angptl4 and PI3K: compared with model group, the BYHWD high and medium-group increased significantly(P<0.05 or P<0.01). CONCLUSION BYHWD can effectively delay denervated tibial muscle atrophy of rats by increasing the wet weight ratio and defering the degeneration of the motor end-plate which mechanism maybe increase Angptl4 and PI3K expression.
Keywords�� buyang huanwu decoction,   denervation,   muscle atrophy,   rat,   tibial muscle,   Angptl4,   PI3K     
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MEI Xiao-Yun, WU Hao-Xin, ZHOU Lan-* .Effect of Buyang Huanwu Decoction on the Denervated Muscle Atrophy of Rats and Investigation for Mechanism[J]  Chinese Pharmaceutical Journal, 2014,V49(9): 726-730
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