目的 对山东德州黄花败酱超临界萃取物进行分析鉴定,测定其抑菌活性及最小抑菌浓度(MIC)。方法 采用超临界CO2萃取法(SFE-CO2)提取黄花败酱的有效成分,用气相色谱-质谱(GC-MS)对其萃取物进行分离鉴定,采用琼脂扩散法测定萃取物的抑菌活性,采用微量稀释法测定其最小抑菌浓度(MIC)。结果 共分离出了24种化学成分,黄花败酱超临界萃取物对沙门氏菌、福氏痢疾杆菌、金黄色葡萄球菌均有明显抑制作用,对大肠杆菌无明显抑制作用,其对福式痢疾杆菌、金黄色葡萄球菌的最小抑菌浓度为4 mg·mL-1,对沙门氏菌的最小抑菌浓度为5 mg·mL-1。结论 为败酱属植物资源的进一步开发应用提供科学而确切的实验依据。
Abstract
OBJECTIVE To extract and analyze the chemical constituents from Patrinia scabiosaefolia Fisch investigate the antibacterial activities and minimum inhibitory concentration (MIC) of the chemical constituents. METHODS The chemical constituents from Patrinia scabiosaefolia Fisch were extracted by SFE-CO2 and analyzed by GC-MS. The antimicrobial activities of the chemical constituents were tested by the agar plate diffusion method. The MIC was obtained by microplate method. RESULTS A total of twenty-four constituents representing 95.407% of the total constituents were identified. The chemical components showed promising antibacterial activity against Staphylococcus aureus, Shigella flexneri and Salmonella spp. The MIC for Staphyloccocus aureus, Shigella flexneri is 4 mg稬-1, and for Salmonella spp, the MIC is 5 mg稬-1. CONCLUSION The experiment provides a scientific basis for further development and utilization of Patrinia scabiosaefolia Fisch.
关键词
败酱草 /
石竹烯 /
植物醇 /
十八烷 /
超临界萃取 /
活性
{{custom_keyword}} /
Key words
Patrinia Scabiosaefolia Fisch. /
caryophyllene /
phytol /
octadecane /
supercritical CO2 fluid extraction /
antibacterial activity
{{custom_keyword}} /
中图分类号：
R284.2
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]YU J Q, LEI J C, YU H, et al. Chemical composition and antimicrobial activity of the essential oil of scuttle lariat barbate. Phytochemistry,2004, 65(23):881-884. [2] GU Z B, CHEN X J, YANG G J, et al. Studies on immunocompetent constituents of Patrinia scabra bunge. J Chin Med Mater (中药材), 2002, 25(3):178-179. [3] JIANG H,CHU Z Y, WANG H X. The constituents of Patrinia scabiosaefolia Fisch . Chin Tradit Herb Drugs (中草药), 2003, 34(11):978-980. [4]LIU G, MA Y M, ZHANG H C. Isolation, identification and antimicrobial activities of endophytic fungi from Cephalotaxus fortunei. Chin Pharm J(中国药学杂志), 2013,48(3):165-170. [5] DONG Y, QI W, XIAO C Y. Analysis of chemical constituents of volatile oils from artemisia mongolica and their antimicrobial activities. Chin Pharm J (中国药学杂志), 2013,48(16):1359-1362. [6] DONG R, NAN M L, LIU H Z. Analysis of chemical composition of leaf volatile oils from two species of ligularia genus from changbai mountain . Food Sci (食品科学), 2010, 31(10):228-230. [7]LIN S J, ZHU X T, JIANG Q Y, et al. Modulation effects of phytol on adipocytes differentiation and glucolipid metabolism . Acta Zoonutrimenta Sin (动物营养学报), 2012, 24(10):1866-1870. [8]BAND N A, JAITA K V, KAUL V K, et al. Synthesis of novel acetates of β-caryophyllene under solvent-free lewis acid catalysis . Nat Prod Res, 2009, 23(15):1445-1450. [9]MALEK S N, LEE G S, HONG S L, et al. Phytochemical and cytotoxic investigations of curcuma mangga rhizomes . Molecules, 2011, 16(6):4539-4548.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
基金
山东省自然科学基金资助项目(ZR2010BL015);山东省新型药用辅料与缓控释工程实验室资助项(13ZX17)
{{custom_fund}}
PDF(600 KB)
