离子色谱法测定灵芝孢子粉中多糖的含量

于青, 尚姝, 冯有龙, 王玉, *

中国药学杂志 ›› 2014, Vol. 49 ›› Issue (4) : 344-347.

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中国药学杂志 ›› 2014, Vol. 49 ›› Issue (4) : 344-347. DOI: 10.11669/cpj.2014.04.020
论 著

离子色谱法测定灵芝孢子粉中多糖的含量

  • 于青1, 尚姝2, 冯有龙2, 王玉1, 2*
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Determination of Monosaccharide Composition of Polysaccharide in Ganoderma lucidum spore by ion Chromatography

  • YU Qing1, SHANG Shu2, FENG You-long2, WANG Yu1, 2*
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摘要

目的 建立一种测定灵芝孢子粉中多糖的单糖组成及含量的方法。方法 采用沸水浴加热提取灵芝孢子粉中多糖, 以2 mol·L-1三氟乙酸(TFA)水解多糖。采用离子色谱法进行测定, 色谱柱为CarboPac PA10(4 mm×250 mm, 10 μm);淋洗液:2.5 mmol·L-1 KOH溶液;柱温30 ℃;流速1.0 mL·min-1结果 9种单糖在一定浓度范围内与色谱峰面积有良好的线性关系, 加标回收率为85.6%~97.0%, RSD均小于3%;灵芝孢子粉中多糖主要由葡萄糖、半乳糖、甘露糖、木糖、岩藻糖等单糖组成。结论 本实验所建立的方法分离效果好, 灵敏度高, 可用于灵芝孢子粉中多糖成分的分析研究。

Abstract

OBJECTIVE To develop a method for determination of monosaccharide composition of Ganoderma lucidum spore polysaccharide. METHODS Ganoderma lucidum spore polysaccharide was extracted in boiling water and hydrolyzed by 2 mol·L-1 TFA. The monosaccharides were determined by ion chromatography on a CarboPac PA10 column, using 2.5 mmol·L-1 KOH as mobile phase. The column temperature was 30 ℃. The flow rate was 1.0 mL·min-1. RESULTSIn the certain range, the concentration of each monosaccharide had good linearity with its chromatographic peak area. The recoveries of monosaccharides ranged from 85.6% to 97.0% with RSDs less than 3%. The RESULTSshowed that Ganoderma lucidum spore polysaccharide was composed of glucose, galactose, mannose, xylose, arabinose, fucose, etc. CONCLUSION The proposed method has good separation effect and high sensitivity, which can be used for the determination of monosaccharide composition of Ganoderma lucidum spore polysaccharide.

关键词

灵芝孢子粉 / 多糖 / 离子色谱法 / 单糖组成 / 含量测定

Key words

Ganoderma lucidum spore / polysaccharide / ion chromatography / monosaccharide composition / content determination

引用本文

导出引用
于青, 尚姝, 冯有龙, 王玉, *. 离子色谱法测定灵芝孢子粉中多糖的含量[J]. 中国药学杂志, 2014, 49(4): 344-347 https://doi.org/10.11669/cpj.2014.04.020
YU Qing, SHANG Shu, FENG You-long, WANG Yu, *. Determination of Monosaccharide Composition of Polysaccharide in Ganoderma lucidum spore by ion Chromatography[J]. Chinese Pharmaceutical Journal, 2014, 49(4): 344-347 https://doi.org/10.11669/cpj.2014.04.020
中图分类号: R917    R284   

参考文献

YAO Z Q, LI W L, ZHOU J, et al. Study on the quality of Ganoderma lucidum spore powder .J Chin Med Mater (中药材), 2007, 30(5):607-611. XIANG J Y, XU A, XIA M, et al. Progress on research of ganoderma polysaccharides . J Pharm Prac(药学实践杂志), 2010, 28(4):241-244. TAN M, QIU X M, LU Y Y, et al. A review on analytical methods for qualification and quantification of plant-derived polysaccharides .Food Sci(食品科学), 2009, 30( 21):420. YU H, MOU S F. Study on factors affecting carbohydrate analysis by high performance anion exchange chromatography-integrated pulsed amperometric detection .J Anal Sci(分析科学学报), 2007, 23(6): 637. STRYDOM D J. Chromatographic separation of 1-phenyl-3-methyl-5-pyrazolone-derivatized neutral, acidic and basic aldoses .J Chromatogr A, 1994, 678:17-23.
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