目的 研究九龙藤总黄酮对缺氧/复氧乳鼠心肌细胞的影响及作用机制。方法 建立心肌细胞缺氧/复氧损伤模型, 给予不同剂量九龙藤总黄酮(终质量浓度分别50、100、200 mg·L-1)预处理, 倒置显微镜下观察心肌细胞形态学变化;酶联免疫法测定肿瘤坏死因子活性;免疫组化方法观察Bcl-2、Bax、NF-κB蛋白的表达;Annex v-FITC/PI双染法测定心肌细胞凋亡率。结果 与模型组相比, 九龙藤总黄酮预处理能减轻心肌细胞损伤, 降低肿瘤坏死因子-α、诱导型一氧化氮合酶、维持内皮型一氧化氮合酶水平, 上调Bcl-2、下调Bax、NF-κβ蛋白表达, 抑制心肌细胞凋亡(P<0.01或P<0.05)。结论 九龙藤总黄酮对缺氧/复氧心肌损伤具有保护作用, 其机制可能与调节诱导型一氧化氮合酶、核转录因子-κβ 信号通路, 上调Bcl-2、下调Bax和核转录因子-κB蛋白表达, 抑制心肌细胞凋亡有关。
Abstract
OBJECTIVE To observe the effect and mechanisms of Bauhinia championii flavones (BCF)on hypoxia/reoxygenation(H/R) myocardial cells of neoanatal rats.METHODS The cardiomyocytes hypoxia/ reoxygenation injury model was developed, and Bauhinia championii flavones was pretreated (final concentration as 50, 100, 200 mg·L-1 respectively). The morphological changes of cardiomyocytes were observed by invert microscope. ELISA was used to evaluate the activities of tumor necrosis factor alpha, the protein expression of Bcl-2, Bax and NF-κβ was observed by immunohisto-chemistry, and Annex v-FITC/PI staining was used to detect apoptosis rate. RESULTS Compared with model group, Bauhinia championii flavones pretreatment eased cardiomyocytes injury, decreased the activities of TNF-α and iNOS, maintained the eNOS level, up-regulated the expression of Bcl-2, down-regulated Bax and NF-κβ, and inhibited cadiocyte apoptosis(P<0.01 or P<0.05). CONCLUSION BCF has protective effects on hypoxia/reoxygenation injury, which are associated with adjusting iNOS and NF-κβ signal channel, up-regulating Bcl-2, down-regulating Bax and NF-κβ, and inhibiting cadiocyte apoptosis.
关键词
九龙藤总黄酮 /
缺氧/复氧损伤 /
心肌细胞
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Key words
Bauhinia championii flavones /
hypoxia/reoxygenation injury /
cradiomyocyte
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中图分类号：
R961
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参考文献
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脚注
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基金
广西自然科学基金资助项目(2012GXNSFAA053148);广西教育厅课题(201103yb094);2013年广西大学生创新创业训练计划立项项目
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