目的 考察O-羧甲基壳聚糖复凝海绵对凝血酶的固定化作用。方法 以水溶性O-羧甲基壳聚糖为载体、戊二醛为交联剂, 加入凝血酶作为复凝血剂, 采用星点设计-效应面法优化O-羧甲基壳聚糖复凝海绵处方, 通过测定固定化凝血酶的活性回收率, 考察凝血酶的固定化条件。结果 O-羧甲基壳聚糖复凝海绵固定凝血酶的最优处方为:O-CMC 2.49%;凝血酶49.87 U·mL^-1; 戊二醛0.49%。结论 O-羧甲基壳聚糖复凝海绵对凝血酶有良好的固定化作用。

OBJECTIVE To investigate the effects of O-carboxymethyl chitosan (O-CMC) multi-hemostatic sponge on the immobilization of thrombin. METHODS The formulation of O-CMC multi-hemostatic sponge was optimized by using central composite design-response surface methodology (CCD-RSM), in which the O-CMC, glutaraldehyde and thrombin were used as enzyme immobilization carrier, crosslink agent and coagulant, respectively. The crosslinked conditions of thrombin were studied by determining the enzymatic activity yield. RESULTS The optimized concentrations of O-CMC, thrombin and glutaraldehyde were 2.49%, 49.87 U穖L^-1, 0.49%, respectively. CONCLUSION O-CMC multi-hemostatic sponge has the positive immobilization effect on thrombin.