目的比较木犀草素对物理性及化学性缺氧/复氧引发H9c2心肌细胞损伤的影响，考察木犀草素对抗两种缺氧/复氧损伤作用。方法体外培养H9c2心肌细胞，以N2饱和缺氧箱及Na2S2O4为缺氧环境分别建立缺氧/复氧损伤模型。测定H9c2心肌细胞存活率、乳酸脱氢酶漏出量、丙二醛的生成量、超氧化物歧化酶以及活性氧的生成量、线粒体膜电位(△Ψm)的变化，考察木犀草素对H9c2心肌细胞缺氧/复氧损伤的影响。结果与模型组比较，木犀草素能显著增强缺氧/复氧损伤心肌细胞存活率，抑制乳酸脱氢酶漏出、丙二醛和活性氧的生成量，提高超氧化物歧化酶的活性(P＜0.05或P＜0.01)，恢复细胞膜电位水平。结论木犀草素对缺氧/复氧心肌细胞损伤有明显的保护作用，能够有效维持抗氧化防御机制与自由基之间的平衡。
Abstract
OBJECTIVE To investigate and compare the effects of luteolin on H9c2 cardiomyocytes damaged by two types of hypoxia/reoxygenation (H/R) injury,and to ensure its protective action against hypoxia/reoxygenation injury. METHODS The H9c2 cardiomyocytes were cultured,and H/R injury model were induced by N2 saturation hypoxia chamber or sodium dithionite (Na2S2O4). In both models,the viability of cardiomyocytes,LDH leakage,MDA levels,T-SOD activities,ROS levels in cells,and mitochondrial membrane potential (△Ψm) changes were assessed to evaluate the effects of luteolin on H/R injury in H9c2 cells. RESULTS Compared with control,luteolin significantly increased cell viability,T-SOD activity in H9c2 cardiomyocytes on H/R injury,and inhibited LDH leakage,MDA and ROS level,and improved △Ψm(P＜0.05 or P＜0.01). CONCLUSION Luteolin has a protective effect against H/R injury in H9c2 cardiomyocytes,and may effectively sustain the balance between anti-oxidant defense and free radicals.
关键词
木犀草素 /
心肌细胞 /
缺氧复氧 /
活性氧
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Key words
luteolin /
cardiomyocytes /
hypoxia/reoxygenation injury /
reactive oxygen species
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中图分类号：
R965
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参考文献
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脚注
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基金
国家“重大新药创制”科技重大专项(2011ZX09401-007)；国家科技支撑计划(2012BAI30B00)；中央高校基本科研业务费专项基金(JKY2009025)
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