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�й�ҩѧ��־ 2013, Vol. 48 Issue (13) :1113-1117    DOI: 10.11669/cpj.2013.13.017
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HUANG Xiang1,WAN Hai-tong1*,ZHANG Heng-yi2,HE Yu1,SUN Yan-wen1,ZHANG Yu-yan1,CAO Yang1,HAN Jin1,HE Chun-xiao1,YANG Jie-hong1,ZHAO Tao3,FU Wei3
1.Zhejiang Chinese Medical University, Hangzhou 310053, China;
2.Zhejiang University,Hangzhou 310053, China;
3.Shandong BUCHANG Pharmaceutical Company Limited,Heze 274000, China

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ժҪ Ŀ�� ����ͬʱ�ⶨ���κ컨��ȡҺ��3����Ч�ɷֵ����ء��ǻ��컨��ɫ��A(HSYA)���Ե����Ậ���ĸ�ЧҺ��ɫ��-���������м����(HPLC-DAD)����;�Ե��κ컨Ϊģ��ҩ��,��΢͸������Ӧ������ҩ����ҩ��ѧ�о�����̽�������� ����΢͸�������͸��Һ,��ЧҺ��ɫ��-���������м����(HPLC-DAD)�ݶ�ϴ�Ѳⶨ͸��Һ�д���ɷֺ���,���㵤�κ컨���������,���쵤���ء��ǻ��컨��ɫ��A���Ե������ڵ�����ȡҺ���컨��ȡҺ�͵��κ컨��ȡҺ͸��ý���л����ʱ仯���,������Ũ�ȡ����ٶԻ����ʵ�Ӱ�졣��� 3�ִ���ɷ���30 min�ڴﵽ���÷���,���Թ�ϵ����(r>0.999 0,n=6)���������ٶ�Ϊ1.0 μL·min-1ʱ,��ϱ�׼Һ�е������ơ��ǻ��컨��ɫ��A���Ե�����Ļ���������Ϊ 11.05%,5.81%,6.73%,����͸��ý������������������,�ض��ɷֵĻ��������½�����,����ͬŨ���»��������������������,����͸��ý���иóɷֵ�Ũ���޹ء����� ΢͸�������������ڵ��κ컨����ҩ��ѧ�о�����ʵ�齨����HPLC-DAD����Ϊ΢͸���о��е��κ컨�����ʵIJⶨ������΢͸��ʵ��Ӧ����Ӱ�����������,ѡ����ʵ�ʵ������,�Է���΢͸����⡣
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Abstract�� Objective To develop an HPLC-DAD method for the simultaneous quantitation of three effective constituents(salvianic acid A, hydroxysafflor yellow A and rosmarinic acid) in Danhong extract; traditional Chinese medicine and taking Danhong as a model drug to explore the feasibility of carrying out pharmacokinetic studies of traditional Chinese medicines by using microdialysis technique. Methods The samples were obtained by microdialysis method and determined by HPLC-DAD gradient elution to calculate the in vitro recovery rate(RR) of specific components. The difference of RRs of salvianic acid A, hydroxysafflor yellow A and rosmarinic acid in the dialysis media of Salvia extract, Safflower extract and Danhong extract, and the effects of flow rate and concentration on RR were studied. Results Good separation of salvianic acid A, hydroxysafflor yellow A and rosmarinic acid was achieved within 30 min, and the three constituents showed good linear relationship(r��0.999 0, n=6). When the flow rate was 1.0 μL·min-1, the RRS of salvianic acid A sodium, hydroxysafflor yellow A and rosmarinic acid in the mixed standard solution were 11.05%, 5.81%, and 6.73%, respectively. Along with the increase of the number of substances in the dialysis medium, the RRs of the specific components showed a downward trend. The RR reduced with increasing flow rate in the same dialysis medium, but was independent of the concentration of the component. Conclusion The microdialysis technique can be used in pharmacokinetic studies of traditional Chinese medicines. The developed HPLC-DAD method in this study can be used for determining the RR of Danhong in the microdialysis study. The factors influencing RR should be considered when choosing appropriate conditions for the microdialysis experiment, thus to make a convenient determination.
Keywords�� danhong extract,   Chinese herbal compound,   microdialysis,   HPLC-DAD     
�ո�����: 2012-11-21;
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������Ȼ��ѧ����������Ŀ(81173647,81274176);�㽭ʡ��Ȼ��ѧ����������Ŀ(Z2101201,LR12H27001);�㽭ʡ�Ƽ�����Ŀ(2012C33122);�㽭ʡ��ҽҩ�ص�ѧ��(2012-XK-A06);�㽭ʡ�����߲�δ����˲�������Ŀ

ͨѶ���� ��ͬ,��,��ʿ,���� �о�������ҽҩѧ�������ٴ��о� Tel/Fax(0571)86613711 E-mailwanhaitong@zjtcm.net     Email: wanhaitong@zjtcm.net
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����, ��ͬ*, �ź����� .��ЧҺ��ɫ��-���������м�����о����κ컨΢͸�������ʵ�����ⶨ��Ӱ������[J]  �й�ҩѧ��־, 2013,V48(13): 1113-1117
HUANG Xiang-, WAN Hai-Tong-*, ZHANG Heng-Yi- etc .In-vitro Microdialysis Recovery of Danhong and Its Influence Factors by HPLC-DAD[J]  Chinese Pharmaceutical Journal, 2013,V48(13): 1113-1117
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[1] CHEN X,LING J J,CHEN L. Study on the in-vitro microdialysis recovery of nicotine and its influence factors. J Guangzhou Univ Tradit Chin Med(������ҽҩ��ѧѧ��),2010,27(2):159-162.
[2] FU X,LING J,WU X. Study on in vitro microdialysis recovery of Shuanghuanglian.China J Chin Mater Med (�й���ҩ��־),2011,36(14) :1951-1954.
[3] WANG D,SHI L F,HU J H. Impact factors on microdialysis probe recovery:Research development. J Int Pharm Res(����ҩѧ�о���־),2008,35(4): 261-265.
[4] ZHANG C,MAO J Y,WANG X L,et al. Systematic review of Danhong Injection for heart failure. China J Tradit Med Pharm(�л���ҽҩ��־),2010,25(12):1956-1960.
[5] ZHU M Z,HU G H,XIAO J. Research progress of application of hydroxysafior yellow A. Chin J Integr Med Cardio/Cerebrovasc Dis(����ҽ�������Ѫ�ܲ���־),2007,5(11):1108-1109.
[6] QI D D,TAN X J,JIA Y,et al.Study on chromatographic fingerprint of Danhong injectable powder. Chin J Pharm Anal(ҩ�������־),2007,27(4):518-521.
[7] ZHANG H Y,SHI H Y,WANG E Y. Effect of environment temperature on the recovery of microdialysis probe for monoamine neurotransmitter. J Zhejiang Univ(Med Sci)(�㽭��ѧѧ��ҽѧ��),2009,38(3):271-275.
[8] YE Y,ZHOU L L,YAN Y L,et al. Study on the in vivo recovery of tetramethylpyrazine phosphate in microdialysis probes. Lishizhen Med Mater Med Res (ʱ���ҽ��ҩ),2009,20(10):2401-2403.
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[2] �̺���Ҧ־����*���� ���������������������Τ׿����Ҧ������.��ҩ�����Ƽ�������ὺ��HPLCָ��ͼ���о�[J]. �й�ҩѧ��־, 2013,48(10): 772-776
[3] ������;�ܸ�;������;���;������;�̱���.΢͸���������۲�ҩ���о��е�Ӧ�ý�չ[J]. �й�ҩѧ��־, 2012,22(9): 657-659
[4] �ź���;������;����ƽ;���㻪.һ�ֻ��ڶ�ͨ��΢͸��������ҩ��С������������������ʵ���·���[J]. �й�ҩѧ��־, 2012,47(8): 653-
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[12] ������ ����ʤ ������ ���� ���� ���� ����.΢͸��������������ܺ��΢�龭�����ǻ��ҩ���԰������о�[J]. �й�ҩѧ��־, 2011,46(24): 1906-1910
[13] ʱ���� ���� ������ �Ͽ�˳ �����.΢͸�����о����������������������ҩ��ѧ[J]. �й�ҩѧ��־, 2011,46(21): 1661-1664
[14] ���� ���ҹ� ���.΢͸�������ϵͳ���õ��о���չ[J]. �й�ҩѧ��־, 2011,46(19): 1457-1460
[15] ���� Ԭ�ٻ� ���� ����.΢͸���������Բ��ŵͪ��ҩ��ҩ������ѧ�е��о���չ[J]. �й�ҩѧ��־, 2011,46(14): 1089-1091
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