Abstract��
Objective To establish an HPLC method for determing irinotecan (CPT-11) and its metabolite 7-ethyl-10 HCPT in rat liver microsome incubation system,and to optimize the incubation conditions. METHODS CPT-11 and SN-38 were determined by HPLC. Single factor design was used to optimize the incubation conditions. RESULTS The linear range of CPT-11 and 7-ethyl-10 HCPT in rat liver microsome incubation system were 20-4 000 ng·mL-1 and 2-400 ng·mL-1,respectively. The optimal incubation conditions were as follows: 10 μmol·L-1 CPT-11,0.02 mg liver microsomes and incubation for 15 min. CONCLUSION The HPLC method is accurate and suitable for the determination of CPT-11 and 7-ethyl-10 HCPT in rat liver microsomes. The incubation condition can be applied in drug interaction studies of irinotecan.
ZHANG Cheng-Liang,
XU Yan-Jiao,
LI Xi-Ping etc
.HPLC Determination of CPT-11 and Its Metabolite 7-ethyl-10 HCPT in Incubation System of Rat Liver Microsomes[J] Chinese Pharmaceutical Journal, 2013,V48(6): 485-488
��
[1]
Pharmacol,2006,57(1):129-133.
[2]
DI PAOLO A,BOCCI G,POLILLO M,et al. Pharmacokinetic and pharmacogenetic predictive markers of irinotecan activity and toxicity . Current Drug Metabolism,2011,12(10):932-943.
[3]
WEI W. Experimental Methodology of Pharmacology (ҩ��ʵ�鷽��ѧ) . Beijing: People’s Medical Publishing House,2010:56-58.
[4]
BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem,1976,72:248-254.
[5]
DENG D,CHEN Q C,LIU T,et al. Study on bincinchonicic acid methodology of detecting protein concentration in liver microsomes of sprague-dawley rats. Pharmacy Today������ҩѧ��,2010,20(9):14-16.
2013, Vol. 48 
