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�й�ҩѧ��־ 2013, Vol. 48 Issue (6) :485-488    DOI: 10.11669/cpj.2013.06.021
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ZHANG Cheng-liang, XU Yan-jiao, LI Xi-ping, WU Tao, LIU Dong*
Department of Pharmacy,Tongji Hospital Affiliated with Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

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ժҪ Ŀ�� ���������΢����ø������ϵ�������濵��CPT-11��������Դ�л����7-�һ�-10�ǻ�ϲ���SN-38����HPLC��ⷽ��,��������������������Ż������� ����HPLC�ⶨ΢����ø������ϵ�������濵�����л�����Ũ��,�õ����ط��Ը��������������Ż�,����Lineweaver-Burk˫�������о�������ø(CES)��ø�ٶ���ѧ����� �����濵��7-�һ�-10�ǻ�ϲ����ֱ���20~4 000��2~400 ng·mL-1�����Թ�ϵ���á�����ø��������Ϊ�������濵 10 μmol·L-1,��΢���嵰�׺���0.02 mg,����ʱ��Ϊ15 min������ ��ʵ�齨����HPLC�ķ�����ݡ�����,�����������濵�����л����IJⶨ������ø�����������Ż�,ͬʱҲΪ�о��������־�������ø��л��ҩ��������濵��лӰ�켰�໥���õ������о��춨�˻�����
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Abstract�� Objective To establish an HPLC method for determing irinotecan (CPT-11) and its metabolite 7-ethyl-10 HCPT in rat liver microsome incubation system,and to optimize the incubation conditions. METHODS CPT-11 and SN-38 were determined by HPLC. Single factor design was used to optimize the incubation conditions. RESULTS The linear range of CPT-11 and 7-ethyl-10 HCPT in rat liver microsome incubation system were 20-4 000 ng·mL-1 and 2-400 ng·mL-1,respectively. The optimal incubation conditions were as follows: 10 μmol·L-1 CPT-11,0.02 mg liver microsomes and incubation for 15 min. CONCLUSION The HPLC method is accurate and suitable for the determination of CPT-11 and 7-ethyl-10 HCPT in rat liver microsomes. The incubation condition can be applied in drug interaction studies of irinotecan.
Keywords�� carboxylesterase ,   incubation system ,   irinotecan ,   7-ethyl-10 HCPT     
�ո�����: 2012-07-16;
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ZHANG Cheng-Liang, XU Yan-Jiao, LI Xi-Ping etc .HPLC Determination of CPT-11 and Its Metabolite 7-ethyl-10 HCPT in Incubation System of Rat Liver Microsomes[J]  Chinese Pharmaceutical Journal, 2013,V48(6): 485-488
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