1 Xinhua Hospital Affiliated to Shanhai Jiaotong University School of Medicine 200092,China; 2 The Graduates Management Brigade of Second Military Medical University,Shanghai 200400,China; 3 Changhai Hospital,Second Military Medical University,Shanghai 200433,China; 4 Shanghai Pulmonary Hospital,Shanghai 200433,China
Abstract��
Objective To investigate the pharmacokinetics of levofloxacin in rats brain tissues and blood��METHODS Brain tissues and blood were sampled by microdialysis simultaneously. The concentrations of levofloxacin in both blood and brain tissues were measured by high performance liquid chromatography��The concentration-time data was analyzed by WinNonlin software��RESULTS The maximum concentrations of free levofloxacin in blood and brain tissues were reached at 10 min and 20 min,respectively,then both continuously decreased��The concentration of free levofloxacin in blood was higher than that in brain tissues in the first 4 h,then returned to similar leve1��The area under the curve (AUC) of free levofloxacin was (21.17±7.83) μg·h·mL-1 in blood and (7.00±2.65) μg·h·mL-1 in brain tissues��CONCLUSION Microdialysis with reversed phase high performance liquid chromatography established in this study can be used to determine the pharmacokinetics of levofloxacin in brain tissues and blood��
ZHOU Jia-,
QIN Sheng-,
XIAO He-Ping- etc
.Pharmacokinetics of Levofloxacin in Rat Brain and Bloods[J] Chinese Pharmaceutical Journal, 2013,V48(6): 466-469
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2013, Vol. 48 
