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�й�ҩѧ��־ 2013, Vol. 48 Issue (6) :461-465    DOI: 10.11669/cpj.2013.06.015
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UPLC-MS/MS��HPLC-UV�ⶨ����ֲ�����󿼷���ѪҩŨ�ȵ�������о�
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CUI Gang, CHEN Wen-qian, LIU Xiao, LI Peng-mei, ZHAO Li, LIU Jun-yang, ZHANG Xiang-lin*
Department of Pharmacy,China Japan Friendship Hospital,Beijing 100029,China

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ժҪ Ŀ�� ����UPLC-MS/MS��HPLC-UV�ⶨ����ֲ�����󿼷��ᣨMPA��ѪҩŨ��,���췽����������, �����ձ����õIJⶨ��������ϵ������ �ֱ����UPLC-MS/MS�ⶨ����ֲ����ȫѪ��Ѫ�����󿼷���Ũ��,HPLC-UV�ⶨ����ֲ����Ѫ�����󿼷���Ũ��,�ⶨ�������LEVENE��LSD�����з������, ��Spearman����������ԱȽϡ���� ����ͳ�Ʒ����õ�3�ַ�����������ʽ,�ֱ�Ϊ��Y����Ѫ��=1.612 X����ȫѪ��0.686;YҺ��Ѫ��=1.414X����ȫѪ�ⶨ-0.206;YҺ��Ѫ��=0.848 X����Ѫ��-0.692������ UPLC-MS/MS�ⶨ����ֲ����ȫѪ��Ѫ���󿼷���Ũ����HPLC-UV�ⶨ����ֲ����Ѫ�����󿼷���Ũ��,3�ַ���Ũ�Ƚ����������õ������, ͨ������ʽ�ɽ��н�����㡣
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Abstract�� Objective To analyze the correlation between UPLC-MS/MS method and HPLC-UV method for determination of mycophenolic acid (MPA) blood concentrations in renal transplant patients and to establish a generally applicable conversion method of the determination results. METHODS MPA concentrations in whole blood and plasma of transplant patients were determined by UPLC-MS/MS method. Plasma concentrations of MPA were determined by HPLC-UV. Variance analysis of the measure results was done by LEVENE and LSD methods,and the correlation between the methods was analyzed by Spearman method. RESULTS Three conversion formula were obtained as follows: YUPLC-MS/MS plasma=1.612 XUPLC-MS/MS whole blood+0.686,YHPLC-UV plasma=1.414XUPLC-MS/MS whole blood-0.206,and YHPLC-UV plasma=0.848 XUPLC-MS/MS whole blood-0.692. CONCLUSION There is good correlation between UPLC-MS/MS method and HPLC-UV method for the determination of MPA concentration in whole blood and plasma in renal transplant patients. The results can be transformed by equations.
Keywords�� mycophenolic acid ,   plasma concentrations ,   UPLC-MS/MS ,   HPLC ,   correlation     
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ͨѶ���� ������,��,����ҩʦ,˶ʿ����ʦ �о������ٴ�ҩѧ������ҩ���� Tel����010��84205370 E-mail��xianglin63@yahoo.comUPLC-MS/MS��HPLC-UV�ⶨ����ֲ�����󿼷���ѪҩŨ�ȵ�������о� �޸�     Email: xianglin63@yahoo.com
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�޸�, ����ٻ, ������ .UPLC-MS/MS��HPLC-UV�ⶨ����ֲ�����󿼷���ѪҩŨ�ȵ�������о�[J]  �й�ҩѧ��־, 2013,V48(6): 461-465
CUI Gang, CHEN Wen-Qian, LIU Xiao etc .A Correlational Study of Three Determination Methods of Mycophenolic Acid Blood Concentrations in Renal Transplant Patients[J]  Chinese Pharmaceutical Journal, 2013,V48(6): 461-465
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