Abstract��
Objective To investigate berberine′s potential to enhance the sensitivity of doxorubicin-resistant human leukemia cell lines (K562/DOX) to doxorubicin in vitro. METHODS MTT assay was performed to determine the effect of berberine on the sensitivity of K562/DOX cells to doxorubicin. Doxorubicin accumulation assay was performed by Arrary Scan VTIHCS600 High-Contents. The effect of berberine on doxorubicin-induced cell apoptosis was tested by PI/Hoechst 33342 assay. The efflux activity of P-gp was investigated by measuring the accumulation of the rhodamine 123 after treatment with berberine. RESULTS 1 μmol·L-1 of berberine,as a wild dose,reduced IC50 of doxorubicin to K562/DOX cells by 1.5 times. 1 μmol·L-1 of berberine showed the enhancement effect on doxorubicin-induced apoptosis. The increase of doxorubicin and rhodamine 123 accumulation was observed in K562/DOX cells treated with 1 μmol·L-1 of berberine which indicated the inhibition of the activity of P-gp. CONCLUSION Berberine is shown to effectively enhance chemosensitivity of K562/DOX cells to Dox by inhibiting the efflux activity of P-gp.
WANG Tian-Xiao-,
WANG Shi-Guang-,
SHI Xiao-Yan- etc
.Effect of Berberine on Sensitivity Enhances of K562/DOX Cells to Doxorubicin[J] Chinese Pharmaceutical Journal, 2013,V48(6): 431-435
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2013, Vol. 48 
