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�й�ҩѧ��־ 2013, Vol. 48 Issue (6) :423-427    DOI: 10.11669/cpj.2013.06.007
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WEI Jin-feng1,2, WANG Jun-xia1, KANG Wen-yi1*
1 Institute of Chinese Materia Medica,Henan University,Kaifeng 475004,China;2 Minsheng College,Henan University,Kaifeng 475004,China

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Abstract�� Objective To study the changes in physiological indices reflecting resistance to environmental stress and volatile constituents of Lysimachia nummularia Aurea. METHODS Different concentrations (150,200,250 and 300 mmol�L-1) of NaCl solution was used to treat young Lysimachia nummularia Aurea to investigate the effect of saline stress on the seedling growth. The changes of physiological indices including soluble sugars,proline,SOD and so on were assayed. Volatile constituents were analyzed by head space solid-phase microextration (HS-SPME) coupled with gas chromatography-mass spectrometry (GC/MS). RESULTS With increasing stress intensity,the contents of soluble proteins declined,those of soluble sugars increased gradually, the content of proline reached the maximum value when saline stress intensity was 250 mmol�L-1,and the contents of chlorophyll a and b decreased firstly and rose later above 250 mmol�L-1. MDA content and SOD activity rose gradually under saline stress with increasing stress intensity. POD activity was significantly lower under the saline stress than the control group. CAT activity rose firstly and rapidly declined later. The volatile constituents of L. nummularia fluctuated after saline stress. CONCLUSION L. nummularia has relatively higher saline resistance by means of active accumulating osmoregulation substances such as soluble sugars,soluble proteins and enhancing capability of antioxidase.
Keywords�� Lysimachia nummularia Aurea ,   NaCl stress ,   physiological indices ,   volatile constituent     
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κ���, , ����ϼ�� .NaClв���½�Ҷ��·������Ҷ��֯�ӷ��Գɷֱ仯����[J]  �й�ҩѧ��־, 2013,V48(6): 423-427
WEI Jin-Feng-, , WANG Jun-Xia- etc .Analysis of Changes in Physiological Indices and Essential oil of Lysimachia nummularia Aurea under NaCl Stress[J]  Chinese Pharmaceutical Journal, 2013,V48(6): 423-427
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[1] LUCZAK S,SWIATEK L,DANIEWSKI M. Phenolic acids in herbs Lysimachia nummularia and L. vulgaris L . Acta Poloniae Pharm,1989,46(4): 381-385.
[2] YASUKAW K,OGAWA H,TAKIDO M. Two flavonol glycosides from Lysimachia nummularia. Phytochemistry,1990,29(5): 1707-1708.
[3] XU G F. Physiological process of drought resistance of two Lysimachia species. J Northwest Forest Univ(������ѧԺѧ��),2007,22(5): 12-14.
[4] LUO J X,SHI Y S,ZHANG X,et al. Identification of cold resistance of 8 ground cover plants by electrical conductivity analysis. J Tianjin Agr Coll(���ũѧԺѧ��),2005,12(3): 10-13.
[5] ZHAO K F,LI F Z,FAN S J,et al. Halophytes in China. Chin Bull Bot(ֲ��ѧͨ��),1999,16(3): 201-207.
[6] LI H S,SUN Q,ZHAO S J,et al. Principles and Techniques of Plant Physiological and Biochemical Experiments(ֲ����������ʵ��ԭ��ͼ���). Beijing: Higher Education Press,2006:164.
[7] ZHANG X Z,SU Z S,FEI X N,et al. Crop Physiology Research Method(�����������). Beijing: Agriculture Press,1992: 211.
[8] XING Y J,CHANG X,KANG W Y,et al. Analysis of volatile compounds from Pimpinella candolleana in Guizhou by SPME-GC-MS. Chin J Exp Tradit Med Form(�й�ʵ�鷽��ѧ),2011,17(4): 93-95.
[9] ZHANG Y F,ZHANG S Q,SHA W, et al. Analysis of physiological changes in seedling of nendan10 of Maize variety under NaCl stress.J Qiqihar Univ(���������ѧѧ��),2006,22(6): 97-99.
[10] WANG L,WU L L,QU Y J,et al. Physiological response and molecular mechanism of puccinellia tenuiflora under NaCl stress. North Horticult(����԰��),2010,23(7): 23-25.
[11] ZHANG L P,WANG X F,SHI Q H,et al. Differences of physiological responses of cucumber seedlings to NaCl and NaHCO3 stress . Chin J Appl Ecol(Ӧ����̬ѧ��),2008,19(8): 1854-1859.
[12] ZHAO F G,SUN C,LIU Y L,et al. Ornithine pathway in proline biosynthesis activated by salt stress in barley seedlings.Acta Bot Sin(ֲ��ѧ��),2001,43(1): 36-40.
[13] WANG L J,LIU Y L,MA K,et al. The role of calcium in promotion of proline accumulation induced by salt stress in Fig (Ficus carica L.) cells. Acta Physiol Sin(ֲ������ѧ��),1999,25(1): 38-42.
[14] WANG J,LI D Q. The accumulation of plant osmoticum and activated oxygen metabolism under stress. Chin Bull Bot(ֲ��ѧͨ��),2001,18(4): 459-465.
[15] NIE J,SU W,CAO Y,et al. The impact of salt stress on the five kinds of green plant chlorophyll content. J Shandong Forest Tech(ɽ����ҵ�Ƽ�),2004,(3): 17-18.
[16] FENG L T,ZHAO K F. Some physiologically adaptive mechanisms of chloroplasts to salt stress. Chin Bull Bot(ֲ��ѧͨ��),1998,15(S1): 62-67.
[17] FENG L T,ZHAO K F. In vivo chlorophyll fluorescence and the screening of salt tolerant crops. J Shandong Normal Univ(ɽ��ʦ��ѧ��),1997,12(4): 436-439.
[18] MI C L,HUANG Z J,SHAO S X,et al. The comparison of chloroplast ultrastructure under salt stress between near isogenic lines in wheat. J Chin Electron Microsc Soc(������΢ѧ��),2001,20(2): 98-101.
[19] LI C Y,HU Y C,NI F T,et al. The growth effect of saline-alkali stress on chloris virgata. Jilin Normal Univ J(����ʦ����ѧѧ��),2008,(4): 24-27.
[20] YANG G H,SHI D C,TIAN W Z,et al. Effects of NaCl and Na2CO3 stress on activity of SOD and POD of Glycyrrhiza uralensis Fisch. Jiangsu Agr Sci(����ũҵ��ѧ),2009,(3): 168-169.
[21] PENG B X,HUANG Z Z,CHEN S H. Determination of chemical constituents in volatile oil from Lysimachia congestiflora Hemsl by GC-MS. Lishizhen Med Mater Med Res(ʱ���ҽ��ҩ),2007,18(10): 2465-2466.
[22] NI S F,FU C X,WU P,et al. The GC-MS research of Lysimachia hemsleyana maximvolatile oil. Chin J Anal Chem(������ѧ�о�����),2004,32(1): 123.
[23] WANG L J,LIU Y L,MA K,et al. The changes of antioxidant enzyme activities of Fig( Ficus carica L.) cell lines with different salt tolerances. Acta Horticulture Sin(԰��ѧ��),1999,26(6):351-355.
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