中国药学杂志
    
           首页  |  期刊介绍  |  编 委 会  |  投稿指南  |  期刊订阅  |  广告服务  |  会议信息  |  联系我们  | 
�й�ҩѧ��־ 2013, Vol. 48 Issue (6) :419-422    DOI: 10.11669/cpj.2013.06.005
��Դ����� ����Ŀ¼ | ����Ŀ¼ | ������� | �߼����� << | >>
�ܹ������չ���������Ҷ���˻��еķֲ�����̬�仯
��ʥ��,л��÷*,��ݼݼ,������
������ҽѧԺ,�ִ���ҩ����ʡ�ص�ʵ����, �Ϸ� 230031
ZHANG Sheng-long, XIE Xiao-mei*, CHENG Jing-jing, SHEN Pan-pan
Anhui College of Traditional Chinese Medicine & Anhui Key Laboratory of Modern Chinese Materia Medica, Hefei 230031,China

Download: PDF (842KB)   HTML (1KB)   Export: BibTeX or EndNote (RIS)      Supporting Info
ժҪ Ŀ�� �����ܹ��ᣨUA������չ���(OA)��������Ҷ����Ҷ�����˻��еķֲ�,��̽����������Ҷ�к����Ķ�̬�仯���ɡ����� ���ó���ЧҺ��ɫ�׷���UPLC���ⶨ�ܹ������չ��Ậ��,ɫ����ΪAcquity BEH C18��2.1 mm×100 mm, 1.7 μm��;������Ϊ�״�-ˮ-������-���Ұ��������Ϊ250��50��0.10��0.05��,���٣�0.25 mL·min-1;��Ⲩ��210 nm,����25 �档��� �ܹ������չ���������Ҷ�к��������������˻�;��Ҷ���ܹ������չ���ĺ���������Ҷ;һ����ÿ���²�ժ������Ҷ���ܹ������չ�����ܺ����仯��Χ��0.874%��0.988%������ �ܹ������չ��������˻���������Ҷ����Ҷ���в�ͬ�ĺ����ֲ�;��ͬ����ʱ������Ҷ�������������������·ݷ���С������,�ò�����Ҫ��Դ�ܹ���;�о����֧��2010��桶�й�ҩ�䡷������Ҷ“ȫ����ɲ���”�Ĺ涨;ͬʱ��ʾ����Ҷ��������Ȼ��Ҷǰ��Ϊ�ѡ�
Service
�ѱ����Ƽ�������
�����ҵ����
�������ù�����
Email Alert
RSS
�����������
��ʥ��
л��÷*
��ݼݼ
������
�ؼ����� ����Ҷ    ���˻�    �ܹ���    ��չ���    ����ЧҺ��ɫ�׷�    �ֲ�    ��̬�仯     
Abstract�� Objective To find out the distribution of ursolic acid and oleanolic acid in fresh leaves,fallen leaves and flowers of Eriobotrya japonica Lindl,and to explore the variation in contents of the triterpene acids in the leaves at different harvest time. METHODS Samples were collected in the campus once every month for 12 months. Determination of ursolic and oleanolic acid was carried out by ultra-high performance liquid cromatography (UPLC) with an acquity BEH C18 column��2.1 mm×100 mm,1.7 μm��using methanol-water-glacial acetic acid-triethylamine��250:50:0.10:0.05��as the mobile phase. The flow rate was 0.25 mL·min-1. The detection wavelength was set at 210 nm. RESULTS The contents of ursolic and oleanolic acids in Erobotrya japonica leaves were significantly higher compared with those in Erobotrya japonica flowers. The contents of the two triterpene acids in the fallen leaves were higher than those in the fresh leaves. The annual curves of dynamic changes of the two triterpene acids contents in Erobotrya japonica leaves showed that the total triterpene acids contents in the Erobotrya japonica leaves collected at the different time varied from 0.874% to 0.988%. CONCLUSION The distribution of the contents of ursolic and oleanolic acid in the fresh leaves,fallen leaves and flowers was different,and there was minor variation in the contents of the two triterpene acids in fresh Erobotrya japonica leaves collected at the different harvest time. This study therefore provides evidence that the Erobotrya japonica leaves included in Chinese Pharmacopoeia (2010 edition,Volume I) can be harvested all the year round. Furthermore,the results suggest that the optimal harvesting time of Erobotrya japonica leaves should be between pre-defoliation period and defoliation period.
Keywords�� Erobotrya japonica leaves ,   Erobotrya japonica flowers ,   ursolic acid ,   oleanolic acid ,   UPLC ,   distribution ,   dynamic changes     
�ո�����: 2012-06-28;
��������:

���ո�Уʡ����Ȼ��ѧ�о���Ŀ��KJ2012A188��

ͨѶ���� л��÷,Ů,����,˶ʿ����ʦ �о�������ҩ�������� Tel/Fax��(0551)65169230 E-mail��xiexiaomei9401@sina.com�ܹ������չ���������Ҷ���˻��еķֲ�����̬�仯 ��ʥ��     Email: zgyxzz@cpa.org.cn
���߼��: ��ʥ��,��,˶ʿ�о��� �о�������ҩ�������� ͨѶ���ߣ�л��÷,Ů,����,˶ʿ����ʦ �о�������ҩ�������� Tel/Fax��(0551)65169230 E-mail��xiexiaomei9401@sina.com
���ñ���:   
��ʥ��, л��÷*, ��ݼݼ�� .�ܹ������չ���������Ҷ���˻��еķֲ�����̬�仯[J]  �й�ҩѧ��־, 2013,V48(6): 419-422
ZHANG Sheng-Long, XIE Xiao-Mei-*, CHENG Jing-Jing etc .Distribution of Ursolic Acid and Oleanolic Acid in the Leaves and Flowers of Erobotrya japonica and Changes of Triterpene Acids Contents in the Leaves at Different Harvest Time[J]  Chinese Pharmaceutical Journal, 2013,V48(6): 419-422
��
[1] Ch. P��2010��Vol���й�ҩ��2010��森һ������2010:190.
[2] WANG J B,YANG B S,ZHANG H,et al��Study on loquat flower extract cough effect ��Chin Tradit Herba Drugs(�в�ҩ),2009,40��7��:1106-1109.
[3] JU J H,ZHOU L,LING G,et al��Studies on constituents of triterpene acids from Eriobotrya japonica and their anti inflammatory and antitussive effects ��Chin Pharm J (�й�ҩѧ��־),2003,38��10��:752-757.
[4] CHA D S,EUN J S,JEON H. Anti-inflammatory and antinociceptive properties of the leaves of Eriobotrya japonica��J Ethnopharm,2011,134��2��:305-312.
[5] LI E N,LUO J G,KONG L Y. Qualitative and quantitative determination of seven triterpene acids in Eriobotrya japonica Lindl. by high-performance liquid chromatography with photodiode array detection and mass spectrometry��Phytochem Anal,2009,20��4��:338-343.
[6] JU J H,ZHOU L,ZHENG R X,et al��Determination of ursolic acid in Eriobotrya japonica by HPLC analysis��Chin Pharm J (�й�ҩѧ��־),2003,38��9��:657-659.
[7] ZHOU C,CHEN K S,SUN C D,et al. Determination of oleanolic acid,ursolic acid and amygdalin in the flower of Eriobotrya japonica Lindl. by HPLC�� Biomed Chromatogr,2007,21��7��:755-761.
[8] XIAO M T,YE J,HUANG Y Y,et al. MECC Determination of ursolic acid and oleanolic acid in leaf of Eriobotrya japonica��Chin J Pharm Anal��ҩ�������־��,2007,27��5��:724-727.
[9] LV M H,XIE X M,ZHA X Z,et al. Determination of oleanolic acid and ursolic acid in the medicinal materials and prepared slices of fructus chaenomelis by reversed phase-high performance liquid chromatography ��J Anhui TCM Coll��������ҽѧԺѧ����,2010,29��3��:72-75.
[10] LV H,XI C P,CHEN J,et al. Determination of triterpenoid acids in leaves of Eriobotrya japonica collected at in different seasons ��China J Chin Mater Med (�й���ҩ��־),2009,34��18��:2353-2355.
[1] ʩ�����򺣿���������*����һ.֬�������ҵ����ڷֲ����ض���������ҵ���������[J]. �й�ҩѧ��־, 2013,48(7): 536-540
[2] �Ȫ�� ���ջ���.����Ҷҩ����������ɷ���ȡ�����о�[J]. �й�ҩѧ��־, 2013,48(10): 805-807
[3] ��ö��;�ľ�;������;������;����;����.��������10����ҩ����15���޻�Ԫ�طֲ����ɼ���������о�[J]. �й�ҩѧ��־, 2012,47(8): 620-625
[4] ������ �ν�.pH����ǰ��������֬������Ʊ������ڷֲ��о�[J]. �й�ҩѧ��־, 2012,47(6): 444-447
[5] ��־�� ���� �ޱ� .�ΰ���������΢��С��������֯�ֲ������о�[J]. �й�ҩѧ��־, 2012,47(5): 354-357
[6] ���ǿ� �·��� ֣��Ȩ ������ .����������ܹ���������ĺϳɼ���θ��ϸ������������[J]. �й�ҩѧ��־, 2012,47(4): 265-269
[7] ��һܲ������ף�������ԣ�����ͮ�����и�.����ЧҺ��ɫ�׷�ͬʱ�ⶨ����Ҷ��ȡ����11�ֻ�ͪ����ɷֵĺ���[J]. �й�ҩѧ��־, 2012,47(24): 2032-2037
[8] �޽࣬ʯ�ܻ��������������񺽣��Ͽ�˳����Ӣ.����ЧҺ��ɫ�׷�ͬʱ�ⶨ֪ĸ��â���յ�6�ֳɷֵĺ���[J]. �й�ҩѧ��־, 2012,47(22): 1856-1859
[9] �������������죬���£�������.��ЧҺ��ɫ�׷��о�3-��������̪�ڴ������ڵ���֯�ֲ����[J]. �й�ҩѧ��־, 2012,47(19): 1574-1576
[10] ������.2005��2010����Ժ�����͸��ײ���������ҩ��Ӧ�÷���[J]. �й�ҩѧ��־, 2012,47(18): 1459-1461
[11] Ф�����������࣬ƽ���ܣ���־��.������A����𤸽������֬��������Ʊ����ڼ����۲���֯�ֲ��о�[J]. �й�ҩѧ��־, 2012,47(15): 1227-1232
[12] ������a����Ӣa*���޽�a����˼��a���ƽ�a���Ͽ�˳b.����ЧҺ��ɫ�׷�ͬʱ�ⶨ������ȥ����Ī���6�ֻ��Գɷֵĺ���[J]. �й�ҩѧ��־, 2012,47(13): 1080-1083
[13] �����;�ŵ���;�ν���;��־��;Ѧ��ƽ.���ִ�Ʒ��Ƽ���С�����ڵ���֯�ֲ��о�[J]. �й�ҩѧ��־, 2012,47(11): 898-902
[14] ������;��퍻�;������;���޽�;����;������.UPLC-Q-TOF-MS�ⶨ�οȴ������˵�����6����Ч�ɷֵĺ���[J]. �й�ҩѧ��־, 2012,47(10): 830-834
[15] ���� �ܰ� ³���� ����ΰ.��ͬ��������������鶯���������պͷֲ��о�[J]. �й�ҩѧ��־, 2012,47(1): 44-48
Copyright 2010 by �й�ҩѧ��־