Abstract��
OBJECTIVE To establish an HPLC-UV method for the simultaneous determination of theophylline and metronidazole in rat plasma, so as to explore the pharmacokinetic interaction between theophylline and metronidazole. METHODS Eighteen male Sprague-Dawley (SD) rats were divided into aminophylline group, metronidazole group, aminophylline and metronidazole group. Blood samples were collected at intervals after each intervenous administration. Then the concentrations of theophylline and metronidazole were determined by HPLC, and the pharmacokinetic parameters were calculated by DAS2.0 software and evaluated by one-way analysis of variance using SPSS19.0 software. Drug-drug interaction was tested by 95% confidence interval. RESULTS Compared with single mediacation groups, the pharmacokinetic parameters t1/2, MRT, AUC0-t��Vd and CL of the combined group showed no significant difference (P > 0.05). CONCLUSION Aminophylline and metronidazole do not have pharmacokinetic interaction in rats.
QI Jing-Jing,
LI Wei,
ZHAO Long-Shan etc
.Pharmacokinetic Interaction between Aminophylline and Metronidazole in Rats[J] Chinese Pharmaceutical Journal, 2013,V48(1): 63-67
��
[1]
NI Y H, ZHU D F, ZHANG H F�� et al. Pharmacodynamics of theophylline in treatment of idiopathic apnea in premature infants . Chin Pharm J (�й�ҩѧ��־), 2011, 46(2):146 -148.
[2]
ZHANG H F, WANG Y, NI Y H�� et al. Relationship of dose and serum concentration of theophylline in 67 cases of premature infant with apnea.Chin Pharm J (�й�ҩѧ��־), 2010, 45(21):1652-1655.
[3]
National pediatric asthma control group. Childhood asthma control routine. Chin J Pedia (�л�������־), 1998, 36(12):747-749.
[4]
CHEN S C, LIU X J. The interaction between aminophylline and commonly used antimicrobial agents. Chin J Clin Rat Drug Use (������ҩ), 2008, 6(2):24-28.
[5]
MA G D, TAN M Y. The effect of metronidazole on aminophylline plasma drug concentration to UV monitoring. Qilu Pharm Aff (��³ҩ��), 1999, 18(2):2.
[6]
ZHU M, SUN Y, GUO D H, et al.Analysis of adverse drug of aminophylline reported in domestic medical journals within past 12 years. Chin J Pharmacoepidemiol (ҩ�����в�ѧ��־), 2006, 15(5):279-280.
[7]
TAYLOR W J. Simkin Handbook of Therapeutic Monitoring. Los Angeles�� SIMKIN Inc, 1991:111.
[8]
QU S W, LI D, WENG Y W. Pharmacokinetic of metronidazole-phosphate and metronidazole in rabbits.Chin Pharm J (�й�ҩѧ��־), 1994, 29(2):101-105.
[9]
JIN M J, WANG M L, YANG C Q.Effect of Nuodikang capsule on pharmacokinetics of theophylline in rats.West China J Pharma Sci (����ҩѧ��־), 2008,23(4):433-435.
[10]
SARKAR M A, HUNT C, GUZELIAN P S�� et al. Characterization of human liver cytochromes P-450 involved in theophylline metabolism. Drug Metab Dispos, 1992, 20��5��:31-37.
2013, Vol. 48 
