目的 建立同时测定大鼠血浆中茶碱、甲硝唑的HPLC-UV分析方法，探索茶碱与甲硝唑在大鼠体内是否存在药动学上的相互作用。方法 将18只雄性SD大鼠随机分成氨茶碱组、甲硝唑组以及氨茶碱和甲硝唑联合给药组，于给药后不同时间点采集血样，HPLC测定二者的血药浓度，采用DAS2.0软件计算药动学参数，并用SPSS19.0软件对其进行单因素方差分析，以95%的可信区间判断药物是否发生相互作用。结果 氨茶碱和甲硝唑联合用药组与单用药组药动学参数t1/2，MRT，AUC0-t，Vd和CL均无显著性差异（P>0.05）。结论 氨茶碱和甲硝唑联合用药后不产生明显的药动学相互作用。
Abstract
OBJECTIVE To establish an HPLC-UV method for the simultaneous determination of theophylline and metronidazole in rat plasma, so as to explore the pharmacokinetic interaction between theophylline and metronidazole. METHODS Eighteen male Sprague-Dawley (SD) rats were divided into aminophylline group, metronidazole group, aminophylline and metronidazole group. Blood samples were collected at intervals after each intervenous administration. Then the concentrations of theophylline and metronidazole were determined by HPLC, and the pharmacokinetic parameters were calculated by DAS2.0 software and evaluated by one-way analysis of variance using SPSS19.0 software. Drug-drug interaction was tested by 95% confidence interval. RESULTS Compared with single mediacation groups, the pharmacokinetic parameters t1/2, MRT, AUC0-t，Vd and CL of the combined group showed no significant difference (P > 0.05). CONCLUSION Aminophylline and metronidazole do not have pharmacokinetic interaction in rats.
关键词
氨茶碱注射液 /
甲硝唑注射液 /
药动学 /
相互作用
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Key words
aminophylline injection /
metronidazole injection /
pharmacokinetics /
interaction
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中图分类号：
R969.1
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参考文献
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脚注
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