摘要
目的 研究益气活血补肾方髓复康对大鼠脑缺血损伤区勿动蛋白（NoGo-A）表达的抑制作用。方法 通过Koizumi法制作SD大鼠单侧大脑中动脉阻塞模型（MACO），将SD大鼠随机分为正常组、模型组、阳性对照组、髓复康大剂量组、中剂量组和小剂量组，各组又分别在给药8、15和30 d 3个时间点取大鼠脑组织，通过免疫组化、RT-PCR的方法检测各组脑缺血损伤区勿动蛋白表达量的差异。结果 中药大剂量及中剂量组勿动蛋白表达最弱，均与模型组形成极显著差异（P＜0.001），小剂量组的勿动蛋白表达也低于模型组，与之形成显著差异（P＜0.05）。结论 髓复康可以抑制脑缺血损伤区勿动蛋白的表达，改善脑缺血损伤区轴突再生微环境，可能是其促进脑缺血损伤后轴突再生的机制之一。
Abstract
OBJECTIVE To study the effect of Chinese herb Suifukang on the expression of Nogo protein-A in the brain of MCAO rats and explore the action mechanism of its promoting neurogenesis. METHODS SD rats were selected and randomized into six groups： normal group， control group， treatment group of methylprednisolone， treatment group of Chinese herb Suifukang in large-does， middle-does and small-does. The models of MCAO rats were successfully established by the method of Koizumi. After the surgery， the rats of drug group had been taken orally Suifukang by 10 g·kg-1 crude drug one time daily， and the rest groups had been taken orally equivalence saline once a day. The brain of rats were investigated and the expression of NoGo-A was detected by immunohistology method and RT-PCR method. RESULTS The expression of NoGo-A in the injured brain was apparently inhibited by Suifukang(P<0.01). CONCLUSION Suifukang can inhibit the expression of NoGo-A. So this function may be one of the mechanisms that Suifukang can promote the functional recovery after brain injury.
关键词
髓复康 /
脑缺血损伤 /
勿动蛋白 /
免疫组织化学
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Key words
Suifukang /
brain injury /
NoGo-A /
immunohistology
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张平，郑巧云，李国辉.
髓复康对大鼠脑缺血损伤区内勿动蛋白（NoGo-A）表达的影响[J]. 中国药学杂志, 2013, 48(1): 35-38
ZHANG Ping，ZHENG Qiao-yun，LI Guo-hui.
Effect of Chinese Herb Suifukang on Expression of NoGo-A Protein in the Brain of MACO Rat[J]. Chinese Pharmaceutical Journal, 2013, 48(1): 35-38
中图分类号：
R965
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参考文献
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脚注
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基金
国家自然科学基金资助项目（30701094）
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