近红外漫反射光谱法快速测定莲房原花青素及多酚含量

周芸，张晓玲，吴永江，刘雪松，栾连军

中国药学杂志 ›› 2013, Vol. 48 ›› Issue (3) : 220-223.

PDF(1031 KB)
中国药学杂志
PDF(1031 KB)
中国药学杂志 ›› 2013, Vol. 48 ›› Issue (3) : 220-223. DOI: 10.11669/cpj.2013.03.013
论 著

近红外漫反射光谱法快速测定莲房原花青素及多酚含量

  • 周芸，张晓玲，吴永江，刘雪松，栾连军
作者信息 +

Determination of Proanthocyanidins and Total Phenolics in the Seedpod of Nelumbo nucifera Gaertn by Near Infrared Diffuse Reflectance Spectroscopy

  • ZHOU Yun, ZHANG Xiao-ling,WU Yong-jiang,LIU Xue-song, LUAN Lian-jun
Author information +
文章历史 +

摘要

目的 建立莲房中原花青素和多酚含量快速测定方法。方法 采用近红外漫反射光谱法（NIR），以紫外-可见分光光度法为对照方法，运用偏最小二乘法（PLS）建立莲房原花青素及多酚含量与近红外漫反射光谱法光谱之间的多元校正模型，实现对原花青素及多酚含量的快速测定。结果 近红外光谱经过二阶导数和Savitzky-Golay平滑滤波处理，选取7 500~6 900 cm-1波段建模得到的模型效果最佳。原花青素校正集相关系数（r）为0.962 1，校正集标准偏差（SEC）为0.64，预测集标准偏差（SEP）为0.94；多酚校正集相关系数（r）为0.925 4，校正集标准偏差为0.97，预测集标准偏差为1.26。结论 该方法快速简便，可用于莲房中原花青素及多酚含量的快速测定。

Abstract

OBJECTIVE To develop a method for rapid determination of proanthocyanidins and total phenolics in the seedpod of Nelumbo nucifera Gaertn by near infrared diffuse reflectance spectroscopy. METHODS Calibration models were established using PLS (partial least squares) method，while the contents of proanthocyanidins and total phenolics were measured by UV. RESULTS The range of 7 500-6 900 cm-1 of near infrared spectra was selected．Different spectra pretreatment methods were compared， and it was shown that second derivative pretreatment and Savitzky-Golay smoothing filter methods could extract spectra information thoroughly. The correlation coefficient (r) of proanthocyanidins was 0.962 1，SEC (standard deviation of the calibration sets) was 0.64， and SEP (standarddeviation of the prediction sets) was 0.94. The correlation coefficient (r) of total phenolics was 0.925 4， SEC was 0.97， and SEP was 1.26. CONCLUSION The near infrared diffuse reflectance spectroscopy method can be used to analyze the main active components in the seedpod of Nelumbo nucifera Gaertn rapidly， so it can be used routinely by the manufacturers for the quality control of the raw materials．

关键词

莲房 / 原花青素 / 多酚 / 近红外光谱

Key words

lotus seedpod / proanthocyanidins / total phenolics / near infrared diffuse reflectance spectroscopy

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用
周芸，张晓玲，吴永江，刘雪松，栾连军. 近红外漫反射光谱法快速测定莲房原花青素及多酚含量[J]. 中国药学杂志, 2013, 48(3): 220-223 https://doi.org/10.11669/cpj.2013.03.013
ZHOU Yun, ZHANG Xiao-ling,WU Yong-jiang,LIU Xue-song, LUAN Lian-jun. Determination of Proanthocyanidins and Total Phenolics in the Seedpod of Nelumbo nucifera Gaertn by Near Infrared Diffuse Reflectance Spectroscopy[J]. Chinese Pharmaceutical Journal, 2013, 48(3): 220-223 https://doi.org/10.11669/cpj.2013.03.013
中图分类号： R282.5   

参考文献

[1] ANDEW K S， HELEN J， ANN C N. Effects of viscosity on the bitterness and astringency of grape seed tannin . Food Qual Prefer， 1996， 7 (9)： 161-166.
[2] ZHANG X H， ZHANG B， GONG B L， et al. Protective effect of procyanidins from the seedpod of the lotus on myocardial ischemia and its mechanisms . Chin Pharm J (中国药学杂志)， 2004， 39(10)： 747-748.
[3] LING Z Q. Study on procyanidins and its biological， pharmacological activities from the seedpod of Nelumbo nucifera Gaertn . Wuhan： Huazhong Agricultural University， 2001.
[4] WANG L L， CHEN C， ZHOU M， et al. Determination of sinigrin in semen thlaspi from Sichuan and tibet using Near Infrared Diffuse Reflectance Spectroscopy . Spectrosc Spectra Anal (光谱学与光谱分析)， 2009， 29 (10)： 2673-2676.
[5] HUANG Q Q， PAN R L， WEI J H， et al. Determination of baicalin and total flavonoids in Radix Scutellariae by Near Infrared Diffuse Reflectance Spectroscopy . Spectrosc Spectra Anal (光谱学与光谱分析)， 2009， 29(9)： 2425-2428.
[6] WANG N， YANG X， ZUO Y， et al. Determination of erythromycin A in erythromycin enteric-coated tables by Near Infrared Diffuse Reflectance Spectroscopy . Chin J Pharm Anal (药物分析杂志)， 2007， 27(3)： 335-338.
[7] DUAN Y Q， ZHANG H H， LI J F， et al. Technology for extracting polyphenols from lotus seedpod by pulsed ultrasonic assisted method . Trans Soc Agric Eng(农业工程学报)， 2009， 25(1)： 193-197.
[8] SUN B S， RICARDO D， SILVA J M， et al. Critical factors of vanillin assay for catechins and proanthocyanidins . J Agr Food Chem， 1998， 46（8）： 4267-4274.
[9] BONOLI M， VERARDO V， MARCONI E， et al. Antioxidant phenols in barley (Hordeum vulgare L.) flour： Comparative spectrophotometric study among extraction methods of free and bound phenolic compounds . J Agr Food Chem， 2004， 52（20）： 5195-5200.
LU W Z， YUAN H F， XU G T. Modern Near Infrared Spectroscopy Analysis Technology (现代近红外光谱分析技术) . Beijing： China Petrochemical Press， 2001： 19.

基金

苏州市科技支撑计划资助项目（ SYN201116）
PDF(1031 KB)

99

Accesses

0

Citation

Detail
段落导航
相关文章

/

〈 〉