Abstract��
OBJECTIVE To develop a method for rapid determination of proanthocyanidins and total phenolics in the seedpod of Nelumbo nucifera Gaertn by near infrared diffuse reflectance spectroscopy. METHODS Calibration models were established using PLS (partial least squares) method��while the contents of proanthocyanidins and total phenolics were measured by UV. RESULTS The range of 7 500-6 900 cm-1 of near infrared spectra was selected��Different spectra pretreatment methods were compared�� and it was shown that second derivative pretreatment and Savitzky-Golay smoothing filter methods could extract spectra information thoroughly. The correlation coefficient (r) of proanthocyanidins was 0.962 1��SEC (standard deviation of the calibration sets) was 0.64�� and SEP (standarddeviation of the prediction sets) was 0.94. The correlation coefficient (r) of total phenolics was 0.925 4�� SEC was 0.97�� and SEP was 1.26. CONCLUSION The near infrared diffuse reflectance spectroscopy method can be used to analyze the main active components in the seedpod of Nelumbo nucifera Gaertn rapidly�� so it can be used routinely by the manufacturers for the quality control of the raw materials��
ZHOU Yun,
ZHANG Xiao-Ling,
WU Yong-Jiang etc
.Determination of Proanthocyanidins and Total Phenolics in the Seedpod of Nelumbo nucifera Gaertn by Near Infrared Diffuse Reflectance Spectroscopy[J] Chinese Pharmaceutical Journal, 2013,V48(3): 220-223
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2013, Vol. 48 