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�й�ҩѧ��־ 2012, Vol. 47 Issue (22) :1849-1855    DOI:
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PAN Shun1��LIU Lei2��FU Xian-shu1��ZHENG Wen-jin1��WANG Wei-min1

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ժҪ Ŀ�� ��ͨ��ɸѡ��������ľù����Trichoderma harzianum��CGMCC No.1780����Һ��peptaibols�࿹���ġ����� ��4��4′-(�Զ��װ���)���������飨TDM����������ɫ�������ٶ��Լ������Զ�����˿�˾���Rhizoctonia solani����˿��������������������׷�٣����ô��������֬���Ͼ�����������Ч��������轺����봿������ľù������Һ��peptaibols�࿹���ģ�����LC-MS/MS����peptaibols�࿹�������нṹ����� �ӹ���ľù������Һ�з����5��peptaibols�࿹���ģ���LC-MS/MS����Ϊ20��peptaibols�����а���5���µ�peptaibols������ Peptaibols�࿹�����ǹ���ľù����Ҫ�������Դ�����л����֮һ��������϶࣬����LC-MS/MS�ɿ�����Ч��������ľù������Һ��peptaibols�࿹���ĵ����нṹ��
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Abstract�� OBJECTIVE To identify peptaibol antibiotics from the fermentation broth of Trichoderma harzianum CGMCC No.1780(China General Microbiological Culture Collection). METHODS Peptaibol antibiotics were isolated using a bioassay-guided fractionation against Rhizoctonia solani. To purify the peptaibols�� the fermentation broth was fractionated by polystyrene resin�� sephadex and high performance thin-layer chromatography (HTLC). A specific chromogenic reaction with 4��4′-methylenebis (N��N-dimethylaniline)was used for the preliminary identification of peptaibols. The sequences of the peptaibol antibiotics were determined by LC-MS/MS analysis.RESULTS Twenty peptaibol antibiotics were identified from the fermentation broth of T. harzianum and five of the peptaibols were novel. CONCLUSION The fermentation broth of T. harzianum is a rich source of peptaibol antibiotics warranting further study and the LC-MS/MS method is rapid and effective to analyze the peptaibols.
Keywords�� Trichoderma harzianum,   peptaibol antibiotics,   LC-MS/MS     
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E-mail�� wwm@cjlu.edu.cn
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PAN Shun, LIU Lei, FU Xian-Shu etc .High Throughput Screening and Detection of Peptaibol Antibiotics from the Fermentation Broth of Trichoderma harzianum by LC-MS/MS[J]  Chinese Pharmaceutical Journal, 2012,V47(22): 1849-1855
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