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�й�ҩѧ��־ 2012, Vol. 47 Issue (19) :1581-1584    DOI:
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1. �㽭��ҽҩ��ѧ��ҩ���Ƽ������ģ� ���� 311401��2. �Ͼ���ҽҩ��ѧҩѧԺ�� �Ͼ� 210046��3. �Ͼ���ҽҩ��ѧ������ʡ��ҩ�����ص�ʵ���ң� �Ͼ� 210049
CAO Gang1��2��CONG Xiao-dong 1��CAI Hao 2��3��CAI Bao-chang 1��2��3
1 Research Center of TCM Processing Technology��Zhejiang Chinese Medical University��Hangzhou 310053��China��2 College of Pharmacy, Nanjing University of Chinese Medicine�� Nanjing 210046��China��3 Jiangsu Key Laboratory of Chinese Medicine Processing��Nanjing University of Chinese Medicine��Nanjing 210029��China

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ժҪ Ŀ�� �����к������˥��ȫ������׼���(attenuated total reflectance��ATR) ��������Ʒ������Ʒ�й��Ͷ��յĺ������п�������IJⶨ������ �Ը�ЧҺ��ɫ��Ϊ���շ����������ⶨ��������ǰ����Ͷ��յĺ���������ƫ��С���˷���partial least squares��PLS������ATR��������Ͷ��յ�HPLC����ֵ֮���ԪУ��ģ�ͣ���������ǰ���δ֪��������Ԥ�⡣��� У��ģ�;���ϵ����ƷΪr2=83.81��SEE��SEP�ֱ�Ϊ0.021 55��0.051 03����ƷУ��ģ�;���ϵ��Ϊr2=95.56��SEE��SEP�ֱ�Ϊ0.007 30��0.034 78�� ���� �÷�������Ϊһ��׼ȷ�����١���Ч������ļ�ⷽ�����ڼ����ҩ����ǰ����Ч�ɷֵı仯���ɡ�
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Abstract�� OBJECTIVE To investigate the feasibility of using mid-infrared spectroscopy (MIRS) technology for rapid quantification of harpagoside contents in crude and processed Radix Scrophulariae by means of attenuated total reflectance. METHODS HPLC was used as the reference method to determine the content of harpagoside��and multivariate calibration models based on PLS algorithm were developed to correlate the spectra and the corresponding values determined by the reference method. RESULTS The determination coefficient of the calibration model for crude Radix Scrophulariae was r2=83.81��and the standard error of estimate (SEE) and the standard error of prediction (SEP) were 0.021 55 and 0.051 03��respectively. The determination coefficient of the calibration model for processed Radix Scrophulariae was r2=95.56��and the standard error of estimate (SEE) and the standard error of prediction (SEP) were 0.007 30 and 0.034 78�� respectively. CONCLUSION The proposed method is fast and nondestructive��and it provides a novel efficient and environmental friendly approach for the fast determination of the active components in crude and processed traditional Chinese medicine (TCM).
Keywords�� attenuated total reflectance,   Scrophulariae Radix,   crude drug(product),   processed drug��product��,   partial least squares,   process analysis     
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�ܸ�, ������, ���� .�����к�����׼������ٲⶨ������Ʒ������Ʒ�й��Ͷ��պ������о�[J]  �й�ҩѧ��־, 2012,V47(19): 1581-1584
CAO Gang, CONG Xiao-Dong, CAI Hao etc .Rapid Determination of Harpagoside Content in Crude and Processed Radix Scrophulariae by Mid-Infrared Spectroscopy[J]  Chinese Pharmaceutical Journal, 2012,V47(19): 1581-1584
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[5] GU W L��CHEN C X��WU Q��et al. Effects of Chinese herb medicine Radix Scrophulariae on ventricular remodeling [J]. Pharmazie��2010��65(10)��770-775.
[6] ZHOU N��BAI Z C. Establishment of the HPLC fingerprint of Radix Scrophulariae [J]. Hubei Agricul Sci������ũҵ��ѧ����2006��45(6)��782-785.
[7] BAI Y��BAO H J��WANG D��et al. The application of infrared spectra and clustering analysis in the discrimination of geographical origin of Flos Chrysanthemic [J]. J Chin Med Mater (��ҩ��)��2006��29(6)��544-548.
[8] HANNEMANN M��ANTUFJEW A��BORGMAN K��et al. Influence of age and sex in exhaled breath samples investigated by means of infrared laser absorption spectroscopy [J]. J Breath Res�� 2011��5(2)��027101.
[9] KHANMOHAMMADI M��GARMARUDI A B��ROUCHI M B��et al. Simultaneous determination of some components in detergent washing powder by mid-infrared spectrometry and artificial neural network[J]. J AOAC Int��2011��94(1)��322-326.
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