基于中红外光谱技术快速测定玄参生品及炮制品中哈巴俄苷含量的研究

曹岗，丛晓东，蔡皓，蔡宝昌

中国药学杂志 ›› 2012, Vol. 47 ›› Issue (19) : 1581-1584.

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中国药学杂志
PDF(841 KB)
中国药学杂志 ›› 2012, Vol. 47 ›› Issue (19) : 1581-1584.
论著

基于中红外光谱技术快速测定玄参生品及炮制品中哈巴俄苷含量的研究

  • 曹岗1，2，丛晓东1，蔡皓2，3，蔡宝昌1，2，3
作者信息 +

Rapid Determination of Harpagoside Content in Crude and Processed Radix Scrophulariae by Mid-Infrared Spectroscopy

  • CAO Gang1，2，CONG Xiao-dong 1，CAI Hao 2，3，CAI Bao-chang 1，2，3
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摘要

目的 采用中红外光谱衰减全反射光谱技术(attenuated total reflectance，ATR) 对玄参生品与炮制品中哈巴俄苷的含量进行快速无损的测定。方法 以高效液相色谱为对照分析方法，测定玄参炮制前后哈巴俄苷的含量，运用偏最小二乘法（partial least squares，PLS）建立ATR光谱与哈巴俄苷的HPLC分析值之间多元校正模型，并对炮制前后的未知样本进行预测。结果 校正模型决定系数生品为r2=83.81，SEE和SEP分别为0.021 55和0.051 03；制品校正模型决定系数为r2=95.56，SEE和SEP分别为0.007 30和0.034 78。 结论 该法可以作为一种准确、快速、高效、无损的检测方法用于检测中药炮制前后有效成分的变化规律。

Abstract

OBJECTIVE To investigate the feasibility of using mid-infrared spectroscopy (MIRS) technology for rapid quantification of harpagoside contents in crude and processed Radix Scrophulariae by means of attenuated total reflectance. METHODS HPLC was used as the reference method to determine the content of harpagoside，and multivariate calibration models based on PLS algorithm were developed to correlate the spectra and the corresponding values determined by the reference method. RESULTS The determination coefficient of the calibration model for crude Radix Scrophulariae was r2=83.81，and the standard error of estimate (SEE) and the standard error of prediction (SEP) were 0.021 55 and 0.051 03，respectively. The determination coefficient of the calibration model for processed Radix Scrophulariae was r2=95.56，and the standard error of estimate (SEE) and the standard error of prediction (SEP) were 0.007 30 and 0.034 78， respectively. CONCLUSION The proposed method is fast and nondestructive，and it provides a novel efficient and environmental friendly approach for the fast determination of the active components in crude and processed traditional Chinese medicine (TCM).

关键词

衰减全反射光谱技术 / 玄参 / 生品 / 炮制品 / 偏最小二乘法 / 过程分析

Key words

attenuated total reflectance / Scrophulariae Radix / crude drug(product) / processed drug（product） / partial least squares / process analysis

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曹岗，丛晓东，蔡皓，蔡宝昌. 基于中红外光谱技术快速测定玄参生品及炮制品中哈巴俄苷含量的研究[J]. 中国药学杂志, 2012, 47(19): 1581-1584
CAO Gang，CONG Xiao-dong ，CAI Hao ，CAI Bao-chang . Rapid Determination of Harpagoside Content in Crude and Processed Radix Scrophulariae by Mid-Infrared Spectroscopy[J]. Chinese Pharmaceutical Journal, 2012, 47(19): 1581-1584
中图分类号： R917   

参考文献

[1] CAI G X，CAI P，ZHANG S H，et al. Determination of harpagoside in Radix Scrophulariae of genuine medical material of hunan with HPLC [J]. Hunan J Tradit Chin Med（湖南中医杂志），2006，22（2）：86-87.
[2] XIAO B M，PEI G，ZENG Y. Determinating cinnamic acid for radix scrophulariae from different districts by HPLC [J]. Chin Arch Tradit Chin Med (中华中医药学刊)，2008，26(1)：117-118.
[3] LI J，LAI D W，SUN W J. HPLC Determination of the contents of four iridiods in Scrophularia ningpoensis Hemsl [J]. Chin J Pharm Anal (药物分析杂志)，2005，25(12)：1531-1533.
[4] YANG X，YANG S P，ZHANG X. Determination of five compounds in Scrophularia ningpoensis by HPLC-UV-ELSD [J]. China J Chin Mater Med (中国中药杂志)，2009，34(1)：68-71.
[5] GU W L，CHEN C X，WU Q，et al. Effects of Chinese herb medicine Radix Scrophulariae on ventricular remodeling [J]. Pharmazie，2010，65(10)：770-775.
[6] ZHOU N，BAI Z C. Establishment of the HPLC fingerprint of Radix Scrophulariae [J]. Hubei Agricul Sci（湖北农业科学），2006，45(6)：782-785.
[7] BAI Y，BAO H J，WANG D，et al. The application of infrared spectra and clustering analysis in the discrimination of geographical origin of Flos Chrysanthemic [J]. J Chin Med Mater (中药材)，2006，29(6)：544-548.
[8] HANNEMANN M，ANTUFJEW A，BORGMAN K，et al. Influence of age and sex in exhaled breath samples investigated by means of infrared laser absorption spectroscopy [J]. J Breath Res， 2011，5(2)：027101.
[9] KHANMOHAMMADI M，GARMARUDI A B，ROUCHI M B，et al. Simultaneous determination of some components in detergent washing powder by mid-infrared spectrometry and artificial neural network[J]. J AOAC Int，2011，94(1)：322-326.
[10] CAO G，CONG X D，CAI H， et al. Simultaneous quantitation of eight active components in crude and processed Radix Scrophulariae extracts by high performance liquid chromatography with diode array detector [J]. Chin J Nat Med (中国天然药物)，2012，10(3)：213-217.

基金

江苏高校优势学科建设工程资助项目，南京中医药大学中药学一级学科开放课题（2011ZYX2-006）；卫生部科研基金（WKJ2010-2-019）；浙江省中医药管理局重点项目（2008ZA002）

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