摘要
目的 探讨板蓝根双糖fructopyrano-（1→4）-glucopyranose（FG）的体内抗肿瘤作用及其对荷瘤小鼠免疫功能的影响。方法 建立小鼠肉瘤S180及小鼠肝癌H22移植性肿瘤模型，观察FG对荷瘤小鼠肿瘤生长的抑制作用；采用腹腔巨噬细胞吞噬实验、淋巴细胞转化实验及Elisa法测定细胞因子IL-2、IL-6、IL-12、TNF-α的水平，检测FG对荷瘤小鼠免疫功能的影响。结果 在50、100、200 mg·kg-1时FG 对S180的肿瘤生长抑制率分别为23.60%、36.76%、49.26%，对H22肿瘤生长抑制率分别为22.61%、36.52%、46.95%，且能提高荷瘤小鼠的脾指数和胸腺指数；FG能增强荷瘤小鼠腹腔巨噬细胞的吞噬功能，促进淋巴细胞转化反应，升高荷瘤小鼠细胞因子IL-2、IL-6、IL-12、TNF-α的水平。结论 FG有较强的体内抗肿瘤活性，其抗肿瘤机制可能与增强荷瘤机体免疫功能有关。
Abstract
OBJECTIVE To study the effects of fructopyrano-（1→4）-glucopyranose（FG） extracted from Radix Isatidis on the tumor growth and immune activity in tumor-bearing mice. METHODS The antitumor activity of FG in vivo was observed by implanted H22 and S180 mouse models. The effect of FG on immune function in tumor-bearing mice was investigated by using phagocytosis test，T plymphocyte proliferation test，and Elisa for determining the expression levels of TNF-α，IL-2，IL-6，and IL-12. RESULTS At dosages of 50，100 and 200 mg·kg-1，the inhibition rates of FG were 23.60%，36.76%and 49.26% for S180，22.61%，36.52%，and 46.95% for H22，respectively. FG increased the spleen index and chest gland index， improved the phagocytosis function of the macrophage and enhance the T lymphocytes proliferation，and increased the expression levels of TNF-α，IL-2，IL-6，and IL-12. CONCLUSION FG exhibits significant anti-tumor effect in vivo and the mechanism may be related to enhancing immune function
关键词
板蓝根 /
fructopyrano-（1→4）-glucopyranose /
荷瘤小鼠 /
免疫功能 /
肿瘤
{{custom_keyword}} /
Key words
Radix Isatidis /
fructopyrano-（1→4）-glucopyranose /
tumor-bearing mice /
immune function /
tumor
{{custom_keyword}} /
刘明华，李茂，孙琴，肖顺汉.
板蓝根双糖fructopyrano-（1→4）-glucopyranose对荷瘤小鼠肿瘤生长及免疫功能的影响[J]. 中国药学杂志, 2012, 47(19): 1542-1546
LIU Ming-huaa，LI Maoa，SUN Qinb，XIAO Shun-hana.
Effects of Fructopyrano-（1→4）-Glucopyranose Extracted from Radix Isatidis on Tumor Growth and Immune Function in Tumor-Bearing Mice[J]. Chinese Pharmaceutical Journal, 2012, 47(19): 1542-1546
中图分类号：
R285
R979.1
{{custom_clc.code}}
({{custom_clc.text}})
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] WIEDENFELD H，ZYCH M，BUCHWALD W， et al. New compounds from Rhodiola kirilowii[J].Sci Pharm， 2007，75：29-34．
[2] XU S Y，BIAN R L，CHEN X. Experimental Methodology of Pharmacology（药理实验方法学）[M]. 3rd ed. BeiJing：People′s Medical Publishing House，2002：1757-1779．
[3] SUN D D，LI X，CHEN J W，et al. Studies on chemical constituents in root of Isatis indigotica I [J].West China J Pharm Sci（华西药学杂志），2007，22（5）：487-488．
[4] BO J，XIAO H，HE J W. Studies on chemical constituents in root of Isatis indigotica [J].China J Chin Mater Med（中国中药杂志），2007，32（3）：271-272．
[5] SHI X，NI H，LIU Y H.Advances in studies on constituents and their pharmacological activities of Isatids Radix[J]. Chin J Hosp Pharm（中国医院药学杂志），2006，26（11）：1397-1399．
[6] XU Y M，LU P C，WANG Y Z. Experimental study on the promotive effect of immune function of Isatidis Radix Polysaccharides [J].Chin J Integr Tradit West Med（中国中西医结合杂志），1991，11（6）：257-358．
[7] FANG J G，LIU Y H， WANG W Q，et al. The anti-endotoxic effect of O-a minobenzoic acid from Radix Isatidis[J]. Acta Pharmacol Sin（中国药理学报），2005，26（5）：593-597．
[8] LIANG Y H，HOU H X，LI D R，et al. Studies on in vitro anticancer activity of tryptanthrin B[J].Chin Tradit Herb Drugs（中草药），2000，31（17）：521-533．
[9] HOU H X，LI D R，QIN J，et al. An experimental study on in - vivo antitumor action of higher unsaturated fatty acids form Radix Isatidis [J].Tradit Chin Drug Res Clin Pharmacol（中药新药与临床药理），2002，13（3）：156-158．
[10] REN M P，LIU M H，LI R，et al. Antitumor activity of astragalus polysaccharides[J]. Chin J New Drugs（中国新药杂志）， 2010，19（3）：221-224.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}
PDF(795 KB)
