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�й�ҩѧ��־ 2012, Vol. 47 Issue (14) :1143-1148    DOI:
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ZHANG Yan-qing

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ժҪ Ŀ�� ����׼ȷ���������ɿ��ĸ�ЧҺ��ɫ��-�������׷� (HPLC-MS/MS)������Ѫ������֯�п�����͡�ĺ��������� ����Agilent C18 (4.6 mm×100 mm��3.5 μm) ɫ��������ˮ-�״� (20��80) Ϊ�����࣬����0.3 mL·min-1�����ײ��õ��������Դ������ģʽ (ESI+)�����ط�Ӧ��� (MRM) ��ʽ�������Ӽ�⣬ҩ�│����͡���ڱ갲��������ڶ�����������Ӧ�ֱ���251.3→206.1��189.1→106.1����������������Ʒ���ô���������ȡ��������� ������͡�ڸ��Բⶨ��Ũ�ȷ�Χ�����Ծ����ã�r > 0.999 0�������ȡ������>60%���С���Ũ�Ⱦ��ܶ�RSD
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Abstract�� OBJECTIVE To establish a highly accurate��sensitive and credible high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the determination of rivastigmine in rat plasma and tissues. METHODS All biological samples were extracted with ethyl acetate. Chromatographic separation was performed on an Agilent C18 column (4.6 mm×100 mm��3.5 μm) using water-methanol (20��80) as mobile phase at a flow rate of 0.3 mL·min-1. The MS analysis was performed by multiple reaction monitoring (MRM) with electronic spray ionization source (ESI+) for quantitative response of rivastigmine (251.3→206) and the internal standard��antipyrine (189.1→106.1) respectively. RESULTS The calibration curves of rivastigmine in plasma and tissues showed satisfactory linearity��and the relative extraction recovery of three concentrations in plasma and all tissues were over 60% except some individuals and all RSDs met the requirements. The limit of quantification of rivastigmine in plasma and tissues were 0.1 and 0.2 ng·mL-1 respectively. CONCLUSION The method is rapid��accurate��highly selective and sensitive��and is suitable for the quantitative analysis of rivastigmine in biological sample of rats.
Keywords�� rivastigmine,   HPLC-MS/MS,   quantitative determination,   plasma,   tissues     
�ո�����: 2011-10-31;
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�����ص�����о���չ�ƻ���Ŀ������2007CB935801����������Ȼ��ѧ����������Ŀ��30970785��

���߼��: �����࣬Ů����ʿ �о�����ҩ���¼��ͺ�ҩ�ﶯ��ѧ ��ͨѶ���ߣ������٣�Ů�����ڣ���ʿ����ʦ �о�����ҩ��ѧ Tel/Fax����010��82801584 E-mail��qixr@bjmu.edu.cn
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������, �����, ��ռ谵� .��ЧҺ��ɫ�״������׷��ⶨ����Ѫ������֯�п�����͡����[J]  �й�ҩѧ��־, 2012,V47(14): 1143-1148
ZHANG Yan-Qing, YANG Zhen-Zhen, WANG Zhan-Zhang etc .Quantitative Determination of Rivastigmine in Rat Plasma and Tissues by HPLC-MS/MS Method[J]  Chinese Pharmaceutical Journal, 2012,V47(14): 1143-1148
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[4] ENZ A��CHAPPUIS A��DATTLER A. A simple��rapid and sensitive method for simultaneous determination of rivastigmine and its major metabolite NAP 226-90 in rat brain and plasma by reversed-phase liquid chromatography coupled to electrospray ionization mass spectrometry [J]. Biomed Chromatogr��2004��18��9����160-166.
[5] SHA Y F��DENG C H��LIU Z��et al. Headspace solid-phase microextraction and capillary gas chromatographic-mass spectrometric determination of rivastigmine in canine plasma samples [J]. J Chromatogr B��2004��806��10����271-276.
[6] LUCANGIOLI S E��TRIPODI V��MASRIAN E��et al. Enantioselective separation of rivastigmine by capillary electrophoresis with cyclodextrines [J]. J Chromatogr B��2005��1081��1����31-35.
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