Abstract��
ABSTRACT��OBJECTIVE To study the purification of the active composition from radix paeoniae rubra��safflower and rhodiola in Qitian Dropping Pill with macroporous resin.METHODS Firstly the kind of resin was screened by using hydroxysafflor yellow A, paeoniflorin and salidroside as index��then, the technical conditions of the enrichment and purification of HPD100 resin were optimized. RESULTS The HPD100 was used for adsorbing hydroxysafflor yellow A and paeoniflorin. The optimal conditions were obtained that the concentrations of sample extract of hydroxysafflor yellow A��paeoniflorin and salidroside was 2.98 g��L-1��1.44 g��L-1��and 2.98 g��L-1 respectively. Absorption flow rate was 60 mL��h-1. Absorption time was above 12h��20% alcohol was as the elution with rate of 60 mL��h-1 and volume was 200 mL. The absorption ability resin was not decreased after being used repeatedly seven times. CONCLUSION The HPD100 is effective for the enrichment and purification of the active composition of radix paeoniae rubra, safflower and rhodiola.
XING Jian-guo,
WANG Xin-chun,
* etc
. Separation and Purification of Main Active Composition of Compound Qitian Dropping Pill by Macroporous Resin[J] Chinese Pharmaceutical Journal, 2009,V44(19): 1470-1474
2009, Vol. 44 
