Abstract��
OBJECTIVE To study the optimal extraction technology of matrine and oxymatrine from Radix Sophorae Tonkinensis.METHODS The total content of matrine and oxymatrine as the inspection index was determined using high performance liquid chromatography��HPLC��.The extraction efficiency of warm immersion extraction��ultrasonic extraction��reflux extraction and accelerated solvent extraction (ASE) was compared.RESULTS Accelerated solvent extraction was superior to the other three methods.The extraction conditions of accelerated solvent extraction were optimized through single-factor test and orthogonal experimental design. The sequence of influential significance was as follows��extraction times��ethanol concentration��extraction temperature��extraction time. The optimal technology of accelerated solvent extraction was that the ethanol concentration was 40%��extraction temperature was 90 �棬the time of extraction was 13 min and the times of extraction was twice.The total content of matrine and oxymatrine from Radix sophorae tonkinensis was (15.527 7��0.431 6) mg��g-1.CONCLUSION The experiment has optimized the extraction method and parameters of alkaloid from Radix sophorae tonkinensis, which provides a new method for the extraction of alkaloid from Radix sophorae tonkinensis.
.Optimization of Extraction of Matrine and Oxymatrine from Radix Sophorae Tonkinensis by Accelerated Solvent Extraction[J] Chinese Pharmaceutical Journal, 2012,V47(7): 495-499
2012, Vol. 47 