布地奈德对哮喘气道重塑大鼠尾加压素-Ⅱ及mRNA表达的影响

张维溪 梁亚峰 聂颖 崇蕾 王小明 李昌崇

中国药学杂志 ›› 2012, Vol. 47 ›› Issue (6) : 427-430.

PDF(1069 KB)
PDF(1069 KB)
中国药学杂志 ›› 2012, Vol. 47 ›› Issue (6) : 427-430.
论著

布地奈德对哮喘气道重塑大鼠尾加压素-Ⅱ及mRNA表达的影响

  • 张维溪,梁亚峰,聂颖,崇蕾,王小明,李昌崇*
作者信息 +

Effect of Budesonide on the Expression of Urotensin-Ⅱ and Its mRNA in Asthma Airway Remodeling Rats

Author information +
文章历史 +

摘要

目的 探讨布地奈德对哮喘气道重塑大鼠尾加压素-Ⅱ(U-Ⅱ)及其mRNA表达的影响。方法 24只清洁级雄性SD大鼠分为3组:对照组、哮喘组和布地奈德组。以卵清白蛋白(OVA)致敏激发法制备哮喘大鼠气道重塑模型,应用图像分析技术测定支气管壁厚度(Wat)和平滑肌厚度(Wam),酶联免疫吸附法(ELISA)测定血清和支气管肺泡灌洗液(BALF)中U-Ⅱ的浓度,免疫组化法和逆转录-聚合酶链反应(RT-PCR)法分别检测U-Ⅱ蛋白和U-Ⅱ mRNA在肺组织中的表达。结果 ①哮喘组Wat和Wam均显著高于对照组,布地奈德组Wat和Wam均显著低于哮喘组(均P <0.01);②哮喘组血清和BALF中U-Ⅱ的浓度均显著高于对照组,布地奈德组上述指标均显著低于哮喘组(均P <0.01);③免疫组化及RT-PCR结果显示,肺组织中U-Ⅱ蛋白及其mRNA的表达,哮喘组显著高于对照组,布地奈德组较哮喘组显著减弱(均P <0.01);④相关性分析显示,大鼠肺组织Wat与U-Ⅱ蛋白、U-Ⅱ mRNA表达呈正相关(均P <0.01),Wam与U-Ⅱ蛋白、U-Ⅱ mRNA表达呈正相关(均P<0.01)。结论 布地奈德对哮喘气道重塑的拮抗作用,可能通过抑制U-Ⅱ的表达起作用。

关键词

尾加压素 / 大鼠 / 哮喘 / 气道重塑 / 布地奈德

引用本文

导出引用
张维溪 梁亚峰 聂颖 崇蕾 王小明 李昌崇 . 布地奈德对哮喘气道重塑大鼠尾加压素-Ⅱ及mRNA表达的影响[J]. 中国药学杂志, 2012, 47(6): 427-430
Effect of Budesonide on the Expression of Urotensin-Ⅱ and Its mRNA in Asthma Airway Remodeling Rats[J]. Chinese Pharmaceutical Journal, 2012, 47(6): 427-430

参考文献


[1] ZHANG W X, LI C C. Airway remodeling: a potential therapeutic target in asthma[J]. World J Pediatr, 2011, 7(2):124-128.
[2] LI C C. The Basic and Clinical of Children Bronchial Asthma(儿童支气管哮喘基础与临床)[M]. Beijing:People′s Medical Publishing House, 2010.
[3] ZHANG W X, LI C C. Airway and children asthma[J].Chin J Pediatr(中华儿科杂志),2006, 44(8): 632-633.
[4] GUAN X J, ZHANG W X, LI C C, et al. The role of external signal regulated kinase and transforming growth factor beta(1) in asthma airway remodeling and regulation of glucocorticoids [J]. Chin Med J(中华医学杂志) ,2007, 87(25): 1767-1772.
[5] LI C C, YE L P, YU X C, et al. Effect of Radix Astragali on signal transducer and activator of transcription 6 and its mRNA expression in a rat asthma model[J].Chin Pharm J(中国药学杂志),2006,41(2):104-107.
[6] BAI A, EIDELMAN D H, HOGG J C, et al. Proposed nomenclature for quantifying subdivisions of the bronchial wall[J]. J Appl Physiol, 1994, 77(2):1011-1014.
[7] COULOUARN Y, LIHRMANN I, JEGOU S, et al. Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord[J]. Proc Nat Acad Sci USA, 1998, 95(26):15803-15808.
[8] RUSSELL F D, MEYERS D, GALBRAITH A J, et al. Elevated plasma levels of human urotensin-Ⅱ immunoreactivity in congestive heart failure [J].Am J Physiol Heart Circ Physiol, 2003,285(4): 1576-1581.
[9] WU Y Q, SONG Z, ZHOU C H, et al. Expression of urotensin II and its receptor in human lung adenocarcinoma A549 cells and the effect of urotensin II on lung adenocarcinoma growth in vitro and in vivo[J]. Oncol Rep, 2010, 24(5): 1179-1184.
[10] KRISTOF A S, YOU Z, HAN Y S, et al. Protein expression of urotensin II, urotensin-related peptide and their receptor in the lungs of patients with lymphangioleiomyomatosis[J].Peptides, 2010, 31(8): 1511-1516.
[11] ZHANG H, ZHANG J B. The correlation research between urotensin and bronchial asthma patients [J].Chin J Tubercul Respirat Dis(中华结核和呼吸杂志), 2003, 26(7): 426.
[12] LI J D, YU Z H, LIU B Y, et al. The changes of urotensin and adrenomedullin in the plasma of bronchial asthma patients and its clinical significance [J]. Chin J Tubercul Respirat Dis(中华结核和呼吸杂志),2003,26(12):797.
[13] BECKETT P A,HOWARTH P H.Pharmacotherapy and airway remodelling in asthma?[J]. Thorax,2003,58(2):163-174.
[14] BOS I S, GOSENS R, ZUIDHOF A B. Inhibition of allergen-induced airway remodelling by tiotropium and budesonide:a comparison[J].Eur Respir J,2007,30(4):653-661.

基金

国家自然科学基金资助项目(30571981);浙江省自然科学基金(Y2090327); 浙江省教育厅项目(Y20070906)
PDF(1069 KB)

28

Accesses

0

Citation

Detail

段落导航
相关文章

/