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�й�ҩѧ��־ 2012, Vol. 47 Issue (5) :380-383     DOI:
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1.Department of Pharmacy�� The Affiliated Hospital of Guiyang Medical Colledge�� Guiyang 550004�� China�� 2. School of Pharmacy�� Guiyang Medical Colledge�� Guiyang 550004��China)

ABSTRACT�� OBJECTIVE To establish a method for simultaneous determination of the contents of muilti-constituents in tall gastrodia capsules for quality control. METHODS The HPLC was performed on a Phenomenex Hydro-RP 80A (4.6 mm��150 mm�� 4 ��m) column with a mixture of methanol-0.1% H3PO4 solution as the mobile phase in a linear gradient elution mode. The flow rate was 1 mL��min-1�� and the column temperature was set at 40 ��. The detection wave length was set at 270 nm. RESULTS The linear ranges were 0.40-6.4 ��g (r=0.999 7) for gastrodin �� 0.064-1.0 ��g(r =0.999 8) for 4-hydroxybenzyl alcohol�� 0.020-0.32��g(r =0.999 7) for 4-hydroxybenzaldehyde �� 0.60-9.5 ��g (r=0.999 9) for parishin�� respectively. The average recovery were 100.8%��RSD 2.3%

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ժҪ Ŀ�� ����ȫ���齺�ҵ��������Ʒ�����������ָ��ɷ�����ȫ���齺�������ķ��������� ����HPLC������Phenomenex Hydro-RP 80A(4.6 mm��150 mm��4 ��m )ɫ������������Ϊ����-0.1%���ᣬ�ݶ�ϴ�ѣ�����1 mL��min-1�����£�40 �棻��Ⲩ����270 nm����� �����ء����ǻ����״������ǻ�����ȩ�Ͱ���ɭ�ս������ֱ���0.40��6.4 ��g(r=0.999 7)�� 0.064��1.0 ��g(r=0.999 8)�� 0.020��0.32 ��g(r =0.999 7)�� 0.60��9.5 ��g(r=0.999 9)���������������Թ�ϵ��ƽ��������(n=9)�ֱ�Ϊ100.8%��RSD 2.3%����100.5%��RSD 3.4%����101.9%��RSD 2.1%����100.4%��RSD 2.5%����25������Ʒ�б���ɷ��������нϺõ�һ���ԣ�RSD 3.4%�������� �÷�������������ȫ���齺�ҵ�������
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Abstract�� OBJECTIVE To establish a method for simultaneous determination of the contents of muilti-constituents in tall gastrodia capsules for quality control. METHODS The HPLC was performed on a Phenomenex Hydro-RP 80A (4.6 mm��150 mm�� 4 ��m) column with a mixture of methanol-0.1% H3PO4 solution as the mobile phase in a linear gradient elution mode. The flow rate was 1 mL��min-1�� and the column temperature was set at 40 ��. The detection wave length was set at 270 nm. RESULTS The linear ranges were 0.40-6.4 ��g (r=0.999 7) for gastrodin �� 0.064-1.0 ��g(r =0.999 8) for 4-hydroxybenzyl alcohol�� 0.020-0.32��g(r =0.999 7) for 4-hydroxybenzaldehyde �� 0.60-9.5 ��g (r=0.999 9) for parishin�� respectively. The average recovery were 100.8%��RSD 2.3%����100.5%��RSD 3.4%����101.9%��RSD 2.1%����100.4%��RSD 2.5%���� respectively. Six constituents were determined. The average content of the 6 constituents of 25 samples were 2.189%��RSD 3.40%��. CONCLUSION The method is reliable and can be used for the quality evaluation of tall gastrodia capsules.
Keywords�� tall gastrodia capsules,   quality evaluation,   HPLC     
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.���ö�ָ��ɷ�����ȫ���齺��Ʒ�ʵ��о�[J]  �й�ҩѧ��־, 2012,V47(5): 380-383
.Quality Evaluation of Tall Gastrodia Capsules by Simultaneous Determination of the Contents of Multi-Constituents[J]  Chinese Pharmaceutical Journal, 2012,V47(5): 380-383
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[2] ���� ������ ���� ���� ŷ���� .�ǻ��컨��ɫ��A���컨��ȡ�P�����Ե���Ƭ���ǻ��컨��ɫ��A�ڴ������ڵ���й�Ƚ��о�[J]. �й�ҩѧ��־, 2012,47(6): 448-453
[3] ������ ��� ������ ������ �̱��� .��ЧҺ��ɫ�׷�ͬʱ�ⶨ�����䲻ͬ����Ʒ��5�ֽ����صĺ���[J]. �й�ҩѧ��־, 2012,47(6): 471-474
[4] ���� ���� ����� ��Ԫ�� ��·ƽ ������ .��é�з�����ɷָ�ЧҺ��ɫ�׷��ⶨ�����Ľ���[J]. �й�ҩѧ��־, 2012,47(5): 375-379
[5] ����Ƽ �ں��� ����� ����ϼ ���ۺ�.����������ƺ����ⶨ�ù��Ҷ���Ʒ������[J]. �й�ҩѧ��־, 2012,47(3): 236-
[6] ���� ����ƽ ֣ӱ.ͨ����ЧҺ��ɫ�ױ���ʱ��ⶨ����޽����-������Ԫ����ˮ����ϵ��[J]. �й�ҩѧ��־, 2012,47(2): 122-126
[7] ������ ����÷ ���� ������ ���ҷ� ʦ�پ�.ע����ͷ����̹�������й����������ڵ�ҩ��ѧ[J]. �й�ҩѧ��־, 2012,47(2): 132-135
[8] �׼��� ��Сƽ ���� Ѧ���� ����.��ЧҺ��ɫ�׷��ⶨ�Ѷ�������Ƥ�ء���ʶ��ء�ɽ����[J]. �й�ҩѧ��־, 2012,47(1): 69-71
[9] ���� ���� ���ж� ����� ʩТ�� ������.��ŵ�������л���HPLC�ⶨ���ڴ���С���ܰ�ת���е�Ӧ��[J]. �й�ҩѧ��־, 2011,46(9): 686-690
[10] ���� �ņ��� ���ٻ� �ſ� ����� ������.������Ƭ�ڽ�������������Ч���о�[J]. �й�ҩѧ��־, 2011,46(9): 695-698
[11] � ���� ������ ����.��ҩ��ɽ�г�ɽ����쳣ɽ��ĺ����ⶨ[J]. �й�ҩѧ��־, 2011,46(8): 623-625
[12] ��Ⱥ ����ƽ �Ϸ� ��� ��ïֲ ����.�����ЧҺ��ɫ�׷��ⶨ��Ѫ��������ͷ����������[J]. �й�ҩѧ��־, 2011,46(7): 545-548
[13] ���� �ν�ƽ ��ѵ�� ������ ������ ����ʥ.�ض��ݻ�ͪ��ɷַ���[J]. �й�ҩѧ��־, 2011,46(7): 542-544
[14] κ���� ����÷ ��� ���� Ѧ��ϼ �Ի���.RP-HPLCͬʱ�ⶨ��������4����Ч�ɷֵĺ���[J]. �й�ҩѧ��־, 2011,46(7): 549-551
[15] Ҧ���� ��С�� �� ������ ������ .RP-HPLC�ⶨ��Ѫ������ƥ��ΤŨ��[J]. �й�ҩѧ��־, 2011,46(6): 463-465
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