中国药学杂志
    
           首页  |  期刊介绍  |  编 委 会  |  投稿指南  |  期刊订阅  |  广告服务  |  会议信息  |  联系我们  | 
�й�ҩѧ��־ 2012, Vol. 47 Issue (4) :300-302     DOI:
���� ����Ŀ¼ | ����Ŀ¼ | ������� | �߼����� << | >>
άC����Ƭ��D-(-)-�쿹��Ѫ��Ľṹ������ɸ��
�ⴺ�����������������º���
����ʡҩƷ������������ 350001
Fujian Institute for Drug Control�� Fuzhou 350001�� China

Download: PDF (749KB)   HTML (0KB)   Export: BibTeX or EndNote (RIS)      Supporting Info
ժҪ Ŀ�� ����άC����Ƭ�зǷ������D-(-)-�쿹��Ѫ��Ľṹ������Ʒ���������ɸ�顣���� ����CAPCELL PAK NH2ɫ������4.6 mm��250 mm��5 ��m����������Ϊ����-20 mmol��L-1����泥������pH��2.5��(60��40)����Ⲩ��Ϊ246 nm������Ϊ1 mL��min-1������ԴΪESIԴ������ѹ��Ϊ220 kPa������������Ϊ12 L��min-1���������¶�350 �棬ëϸ�ܵ�ѹ3.5 kV�������Ӽ�⡣��Һ��ɫ�׷��벢�Ʊ�άC����Ƭ����������13C-NMR�ṹȷ֤����� ͨ���Լ���������[M+H]+�༶�����ѽ���ɽ�������D-(-)-�쿹��Ѫ�����Ʒ�Ƚϣ����������ΪD-(-)-�쿹��Ѫ�᣻ͨ�������13C-NMR��ѧλ�Ʒ�������һ��ȷ����ṹ����366��άC����Ƭ��Ʒ�У���64����Ʒ����Ƿ������D-(-)-�쿹��Ѫ�ᡣ���� Һ�����÷���׼ȷ�������ȸߣ������㶨�Լ���Ҫ��
Service
�ѱ����Ƽ�������
�����ҵ����
�������ù�����
Email Alert
RSS
�����������
�ؼ����� άC����Ƭ   D-(-)-�쿹��Ѫ��   �ṹ����   ɸ��     
Abstract�� OBJECTIVE To identify the structure of illegal additive D-(-)-isoascorbic acid in vitamin C Yinqiao tablets and to screen for it in different samples. METHODS The separation of D-(-)- isoascorbic acid in vitamin C Yinqiao tablets was performed on a CAPCELL PAK NH2 column (4.6 mm��250 mm��5 ��m) with mobile phase consisting acetonitrile -20 mmol��L-1 ammonium acetate (adjusted to pH 2.5 with formic acid) (60��40)�� the flow rate was 1.0 mL��min-1. D-(-)- isoascorbic acid was detected by diode array detector at 246 nm. Electrospray ionization (ESI) source in positive ion mode was applied for the identification with following parameters�� nebulizer pressure of 220 kPa�� drying gas temperature of 350 �棬 flow rate of 12 L��min-1�� and capillary voltage of 3.5 kV. The additive D-(-)- isoascorbic acid in vitamin C Yinqiao tablets was separated and prepared by HPLC for further structure confirmation by 13C-NMR. RESULTS The structure of the additive was elucidated by analyzing the fragment of [M+H]+ of the additive and comparing with that the reference substance. The additive was further confirmed as D-(-)- isoascorbic acid by 13C-NMR . D-(-)- isoascorbic acid was detected in 64 of 366 batch samples. CONCLUSION The LC-MS method is accurate and sensitive�� which can be used for rapid screening and identification of the illegal additive D-(-)- isoascorbic acid in vitamin C Yinqiao tablets.
Keywords�� vitamin C Yinqiao tablets,   D-(-)- isoascorbic acid,   structure identification,   screening     
�ո�����: 2011-07-29;
���߼��: �ⴺ����Ů����ʿ������ҩʦ��˶ʿ����ʦ �о�������ҩ��Ч�ɷַ������������� Tel�� (0591)87622202 E-mail�� wchmra@yahoo.com.cn
���ñ���:   
.άC����Ƭ��D-(-)-�쿹��Ѫ��Ľṹ������ɸ��[J]  �й�ҩѧ��־, 2012,V47(4): 300-302
.The Structure Identification and Screening of Illegal Additive D-(-)- Isoascorbic Acid in Vitamin C Yinqiao Tablets[J]  Chinese Pharmaceutical Journal, 2012,V47(4): 300-302
��
û�б��IJο�����
[1] ��С�� ������ ������ �鿡�� �ֱ�� ����� .����ޣ�Ļ�ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2012,47(6): 415-417
[2] ������ ���� ֣���� �Ŵ��� ���.��������ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2011,46(5): 338-340
[3] κ���� ��⻪ �ܳ�÷ �ܵ��� ������ ����� .������ëާ�л�ͪ�໯ѧ�ɷֵ��о�[J]. �й�ҩѧ��־, 2011,46(3): 177-179
[4] ʯ�� ������ ³���� ���� ����ë.������߾�Streptomyces sp. LZ35�еİ�ɯù���໯����[J]. �й�ҩѧ��־, 2011,46(17): 1317-1320
[5] ϯ���� ����ɼ ���� ������ ���� �Ŵ���.�ڴ����ָ���ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2011,46(13): 980-983
[6] ����� ɳ���� ��÷��.�����μ�����ѧ�ɷֵ��о�����[J]. �й�ҩѧ��־, 2011,46(1): 11-13
[7] ������ ��ٻ ������ ���˴�.3�ֺ���Ŀ�����ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2010,45(5): 332-334
[8] ������ ���� ��ѩ�� ������ ������.�Ϻ�������������ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2010,45(5): 339-340
[9] ��ޱ ���ɽ.ľ���׾���ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2010,45(20): 1528-1529
[10] ��½ ��Ծ�� ������ ������ ��ʤ�� �׵���.���һ���ѧ�ɷ��о� [J]. �й�ҩѧ��־, 2010,45(14): 1054-1056
[11] ������ ���� ֣�� �ņ� ���� ����Ȫ.����ɫ�׷�����ɸ�齵֬����ѹ��ֹ��ƽ������ҩ�Ƽ��е�29����ӻ�ѧҩ��[J]. �й�ҩѧ��־, 2010,45(11): 857-861
[12] Ԭʤ�� ������ Ϳ��÷ Ϳ���� ���� ����.�䵱��ţ����ѧ�ɷ����־����Եij����о�[J]. �й�ҩѧ��־, 2010,45(10): 733-735
[13] ����ȫ���뱦�� ��ʷ��ӱ�����ᣬ������ . ݡ����л�ѧ�ɷֵ��о�[J]. �й�ҩѧ��־, 2009,44(18): 1372-1374
[14] ������ ������ ���߻� �������� ����ϳ . ¹���Ļ�ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2009,44(17): 1287-1290
[15] �Ź��;����;��Ԫ��;��ϳ;����;������.���մ̵Ļ�ѧ�ɷ��о�[J]. �й�ҩѧ��־, 2009,44(12): 897-899
Copyright 2010 by �й�ҩѧ��־