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�й�ҩѧ��־ 2012, Vol. 47 Issue (4) :279-282     DOI:
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1. �㶫ҩѧԺҩ��ѧԺ������ 510006��2. �㶫ʡҩ���¼����ص�ʵ���ң����� 510006
1. School of Pharmacy�� Guangdong Pharmaceutical University�� Guangzhou 510006�� China; 2. Guangdong Provincial Key Laboratory of Advanced Drug Delivery�� Guangzhou 510006�� China

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ժҪ Ŀ�� �о�ӫ��̽�뾭�ڶ���ҩ���ת��ͨ·������ ���㶹��-6Ϊӫ��̽�룬�����黯-�ܼ��ӷ����Ʊ�����/�ǻ����Ṳ���PLGA�������������������ע��ֱ�������������Һ�����������ڶ����ڲ�ͬʱ���ɼ���ҩ�����ܰ�Һ��PL�����Բ�PL���Լ�Һ��CSF����Ѫ�������� HPLC�ⶨ����֯���㶹��-6��Ũ�ȣ�������ҩ��ѧ��������� �������ע������������Һ�����㶹��-6�ڸ�ҩ��PL���Բ�PL��CSF�о��зֲ�������Һ����ȣ�����ע�����������㶹��-6�ڸ�ҩ��PL��CSF��AUC�ֱ�����3.7��7.6������max�ֱ����9.9��9.0��������MRT �ֱ��ӳ�5.1��9.4�������� �ڶ���ҩ��ת��;��Ϊ��ҩ��PL��CSF���Բ�PL�����Ҳ�����������ҩ����������ڶ���CSF�ľֲ��������öȣ��ӳ�����ʱ�䡣
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Abstract�� OBJECTIVE To investigate transport route of fluorescent probe following inner ear administration. METHODS Coumarin-6 (CM) was employed as fluorescent probe and CM-loaded poly(lactic-co-glycolic acid) nanoparticles (CM-PLGA-NP) were prepared by emulsification-solvent evaporation method. Perilymph (PL)�� contralateral PL�� cerebrospinal fluid (CSF) and plasma were collected periodically in guinea pigs after unilateral intratympanic administration of CM-PLGA-NP or CM solution. The concentrations of CM were determined by high performance liquid chromatography (HPLC)�� and statistic program DAS was applied to the calculation of pharmacokinetic parameters. RESULTS CM was found in PL�� contralateral PL and CSF after unilateral intratympanic administration of CM-PLGA-NP or CM solution. The AUC in PL and CSF following intratympanic administration of CM-PLGA-NP were respectively 3.7 and 7.6-fold higher than those following intratympanic administration of CM solution. And the ��max in PL and CSF were respectively 9.9 and 9.0-fold higher. Moreover�� CM-PLGA-NP extended the MRT in PL and CSF by 5.1 and 9.4 times compared with CM solution. CONCLUSION Transport pathway of intra-cochlear administration is PL��CSF��contralateral PL. Nanoparticles can greatly improve drug distribution within the inner ear and CSF�� and prolong the residence time.
Keywords�� coumarin-6,   intra-cochlear administration,   transport route,   PLGA nanoparticle     
�ո�����: 2011-08-03;
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.����ӫ��̽���ڶ���ҩת��ͨ·�Ŀ���[J]  �й�ҩѧ��־, 2012,V47(4): 279-282
.Transport Route of Fluorescent Probe via Inner Ear Administration[J]  Chinese Pharmaceutical Journal, 2012,V47(4): 279-282
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[1] CHEN G�� ZHANG X�� YANG F�� et al. Disposition of nanoparticle-based delivery system via inner ear administration[J]. Curr Durg Metab�� 2010�� 11(10)�� 886-897.[2] CHEN G�� HOU S X�� HU P�� et al. Preliminary study on brain-targeted drug delivery via inner ear[J]. Acta Pharm Sin��ҩѧѧ������ 2007�� 42(10)�� 1102-1106.[3] CHEN G�� MU L�� LU C Y�� et al. Preliminary study on brain-targeted delivery of human interferon ��2b following intratympanic administration[J]. Chin Pharm J ���й�ҩѧ��־���� 2010�� 45(24)�� 1922-1925.[4] CHEN G�� MU L�� ZHANG X�� et al. In vivo distribution and pharmacokinetics of multiple effective components contained in panax notoginseng saponins after intratympanic administration[J]. China J Chin Mater Med���й���ҩ��־���� 2011�� 36(13)�� 1815-1820.[5] GAO X L. Lectin-conjugated nanoparticles for drugs delivery into brain following intranasal administration[D]. Shanghai�� Fudan University�� 2007.[6] HU K L�� LI J W�� SHEN Y H�� et al. Lactoferrin-conjugated PEG-PLA nanoparticles with improved brain delivery�� In vitro and in vivo evaluations[J]. J Controlled Release�� 2009�� 134(1)�� 55-61.[7] LI X F�� MA Y J��ZHANG X��et al. Preparation of fluorescent probe coumarin-6 loaded PLGA nanoparticles[J]. Chin J Mod Appl Pharm���й��ִ�Ӧ��ҩѧ���� 2011�� 28(8)�� 740-744.[8] WANG Y. Origin�� character and composition of lymph of inner ear[J]. Bull Biol������ѧͨ������ 2006�� 41(12)�� 23.[9] KELLERHALS B. Perilymph production and cochlear blood flow[J]. Acta Otolaryngol�� 1979�� 87(3-4)�� 370-374.[10] GALLE E�� SIEGEL G. The permeability of 51Cr between cerebrospinal fluid and internal ear[J]. Arch Otorhinolaryngol�� 1979�� 224(1-2)�� 85-88.[11] GOYCOOLEA M V�� LUNDMAN L. Round window membrane. Structure function and permeability�� a review[J]. Micros Res Tech�� 1997�� 36(3)�� 201-211.
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