目的 建立中药材中4种黄曲霉毒素测定的快速液相色谱-串联质谱方法。方法 样品经体积分数70%甲醇提取、免疫亲和柱净化，岛津Shim-pack XR-ODS色谱柱，用甲醇-乙腈（1∶1）和水为流动相，梯度洗脱，ESI扫描，多反应监测（MRM）。结果 黄曲霉毒素B₁在0.102 1~10.21 ng·mL内、黄曲霉毒素B₂在0.061 2~7.65 ng·mL内、黄曲霉毒素G₁在0.193~9.65 ng·mL内、黄曲霉毒素G₂在0.121~7.55 ng·mL内，线性关系均良好，r＞0.999 8；4种黄曲霉毒素回收率在77.0％~102.4％之间。结论 本方法准确可靠，适合中药材中4种黄曲霉毒素含量的测定。

OBJECTIVE To develope a method for determination of four aflatoxins (B₁， B₂， G₁， G₂) in fruit traditional Chinese medicines by rapid-resolution liquid chromatography coupled with electrospray ionization triple quadrupole tandem mass spectrometry. METHODS After being extracted with 70% methanol and purified by immunoaffinity columns， the four aflatoxins were separated on a Shim-pack XR-ODS column using gradient elution with the mobile phase of CH₃CN-CH₃OH-H₂O mixtures monitored under MRM mode in ESI positive scan. RESULTS There was a good linear relationship over the range of 0.102 1-10.21 ng·mL for AFB₁， 0.061 2-7.65 ng·mL for AFB₂， 0.193-9.65 ng·mL for AFG₁， 0.121-7.55 ng·mL for AFG₂ (r＞0.999 8)， respectively. The average recoveries ranged from 77.0%-102.4%. CONCLUSION This method provides a simple and feasible way to determine aflatoxins.