目的 研究对比疫苗中蔗糖检验的3种仪器分析方法。方法 建立了基于比旋度、高效液相色谱-示差折光检测法和离子色谱-积分脉冲安培检测法对疫苗制品中蔗糖进行质控分析的方法。结果 在选定的分析条件下，比旋度、高效液相色谱-示差折光检测法和离子色谱-积分脉冲安培检测法均能准确给出疫苗制品中蔗糖的含量。结论 比旋度分析在没有背景干扰的情况下简单易行，高效液相色谱-示差折光检测法和离子色谱-积分脉冲安培检测法则具有更强的专属性及更高的准确度。

OBJECTIVE To study three instrumental analysis methods of sucrose in vaccine product.METHODS The quality control methods of sucrose were performed by specific optical rotation， high performance liquid chromatography-refractive index detection (HPLC-RID) and ion chromatography-integrated pulsed amperometric detection(IC-IPAD). RESULTS Under the selected conditions， specific optical rotation， HPLC-RID and IC-IPAD methods provided accurately the concentration of sucrose in vaccine product. CONCLUSION Specific optical rotation is easy to implement when matrix interferences don′t influence the assay. However， HPLC-RID and IC-IPAD methods are more specification and accuracy for the identification and quantitative analysis of sucrose in vaccine product.