摘要
目的 考察苦参碱(matrine,Mat)对皮肤角质形成细胞(keratinocytes, KC)系HaCaT细胞白细胞介素-17受体(IL-17R)、p-p38 MAPK表达的影响,及其对HaCaT细胞分泌白细胞介素(IL)-6、IL-8、MIP-3α等炎症因子的影响。方法 采用RT-PCR方法检测Mat对皮肤KCIL-17R mRNA表达的影响;采用流式细胞术检测Mat对HaCaT细胞IL-17R蛋白含量的影响;采用Western blot方法检测Mat对HaCaT细胞表达p-p38 MAPK的影响;ELISA法检测Mat对HaCaT细胞分泌炎症因子IL-6、IL-8及MIP-3α的影响。结果 Mat可降低HaCaT细胞表达IL-17R和p-p38 MAPK,以及降低HaCaT细胞IL-6、IL-8、MIP-3α等炎症因子的分泌。结论 Mat可通过抑制IL-17R的表达,阻止p38 MAPK的磷酸化过程而具有抗炎作用。
Abstract
OBJECTIVE To investigate the effect of matrine on the expression of IL-17R, p-p38 MAPK in skin keratinocyte cell line HaCaT cells, and on the level of IL-6, IL-8, MIP-3α secreted by HaCaT cells. METHODS RT-PCR method was applied to analyze the expression of IL-17R mRNA in HaCaT cells treated with matrine. Flow cytometry was used to analyze the IL-17R protein content. The expression of p-p38 MAPK was determined by Western blot method. The levels of inflammatory factors IL-6, IL-8 and MIP-3α were analyzed by ELISA. RESULTS Matrine decreased the expression of IL-17R and p-p38 MAPK in HaCaT cells, and the level of IL-6, IL-8 and MIP-3α secreted by HaCaT cells. CONCLUSION Matrine can inhibit the expression of IL-17R to prevent the phosphorylation of p38 MAPK, and inhibit the expression of IL-6, IL-8, MIP-3α, and so matrine has anti-inflammatory effect.
关键词
苦参碱 /
白介素-17受体 /
磷酸化p38促有丝分裂原活化蛋白激酶 /
白介素-6 /
白介素-8 /
巨噬细胞炎性蛋白3α
{{custom_keyword}} /
Key words
matrine /
interleukin-17 receptor /
p-p38 MAPK /
interleukin-6 /
interleukin-8 /
MIP-3α
{{custom_keyword}} /
孙创斌 胡晋红 朱全刚 刘继勇 刘艳霞 沈奎亚.
苦参碱对皮肤角质形成细胞IL-17R、p-p38 MAPK和炎症因子的影响[J]. 中国药学杂志, 2010, 45(7): 506-509
SUN Chung-in;HU Jin-hong;ZHU Qun-gng;LIU Ji-yong;LIU Yn-xi;SHEN Kui-y.
Effects of Matrine on IL-17R, p-p38 MAPK and Inflammatory Factors in Cultured Keratinocytes[J]. Chinese Pharmaceutical Journal, 2010, 45(7): 506-509
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] KUMAGAI N, FUKUDA K, FUJITSU Y, et al. Expression of functional ICAM-1 on cultured human keratocytes induced by tumor necrosis factor-alpha[J]. Jpn J Ophthalmol, 2003,47 (2):134-141.
[2] YU B, KOGA T, URABE K, et al. Differential regulation of thymus-and activation-regulated chemokine induced by IL-4, IL-13, TNF-αlpha and IFN-gamma in human keratinocyte and fibroblast[J]. J Dermatol Sci, 2002,30 (1):29-36.
[3] HERLAAR E, BROWN Z. p38 MAPK signalling cascades in inflammatory disease[J]. Mol Med Today, 1999, 5(10):439-447.
[4] WEAVER C T, HARRINGTON L E, MANGAN P R, et al. Th17: an effector CD4+ T cell lineage with regulatory T cell ties[J]. Immunity, 2006, 24(6):677-688.
[5] WEI S, SIEGAL G P. p38 MAPK as a potential therapeutic target for inflammatory osteolysis[J]. Adv Anat Pathol, 2007, 14(1):42-45.
[6] NICKOLOFF B J, MITRA R S, GREEN J, et al. Activated keratinocytes present bacterial-derived superantigens to T lymphocytes: relevance to psoriasis[J]. J Dermatol Sci, 1993, 6(2):127-133.
[7] ZBYTEK B, SLOMINSKI A T. CRH mediates inflammation induced by lipopolysaccharide in human adult epidermal keratinocytes[J]. J Invest Dermatol, 2007, 127(3):730-732.
[8] WARNER R L, BHAGAVATHULA N, NERUSU K C, et al. Matrix metalloproteinases in acute inflammation: Induction of MMP-3 and MMP-9 in fibroblasts and epithelial cells following exposure to pro-inflammatory mediators in vitro[J]. Exp Mol Pathol, 2004,76 (3):189-195.
[9] LI D, WANG Q P. Study and clinic application of matrine[J]. Chin Tradit Her Drugs(中草药),1996,27(5):308-309.
[10] ZHANG J P, ZHANG M, ZHOU J P, et al. Antifibrotic effects of matrine on in vitro and in vivo models of liver fibrosis in rats[J]. Acta Pharmacol Sin(中国药理学报), 2001, 22(2):183-186.
[11] BORDEN E C, CHIN P. Interleukin-6: a cytokine with potential diagnostic and therapeutic roles[J]. J Lab Clin Med, 1994, 123(6):824-829.
[12] NICKOLOFF B J. The cytokine network in psoriasis[J]. Arch Dermatol, 1991,127:871-884.
[13] HOMEY B, DIEU-NOSJEAN M C, WIESENBORN A, et al. Up-regulation of macrophage inflammatory protein-3 alpha/CCL20 and CC chemokine receptor 6 in psoriasis[J]. J Immunol, 2000, 164(12):6621-6632.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}