Effects of Total of Flacone C on the Cell Apoptosis in Rats with Focal Cerebral Ischemia-Reperfusion Injury
LUO Sheng-yong， FANG Ming， CHEN Zhi-wu
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Department of Pharmacology， Anhui Medical University， Hefei 230032， China
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出版日期
2012-01-01
2012-01-01
2010-04-10
发布日期
2010-05-05
摘要
lsy770728@126.com
Abstract
OBJECTIVE To study the effects of total of flacone C |TFC) on the cell apoptosis in rats with focal cerebral ischemia-reperfusion injury. METHODS Using modified longa method the model of rat focal cerebral I/R injury|2 h ischemia and 2 h reperfusion ）was established. After operation， the rats were sacrificed and brains were removed for the detection of apoptotic neurons by the method of TUNEL and transmission election microscope. RESULTS TFC can significantly reduced the numbers of dead neural cells， restrained the cell apoptosis after focal cerebral ischemia-reperfusion injury .CONCLUSION TFC can remarkably restrain the cell apoptosis after focal cerebral ischemia-reperfusion injury.
LUO Sheng-yong;FNG Ming;CHEN Zhi-wu.
Effects of Total of Flacone C on the Cell Apoptosis in Rats with Focal Cerebral Ischemia-Reperfusion Injury[J]. Chinese Pharmaceutical Journal, 2010, 45(9): 665-668
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参考文献
［1］ LUO S Y，DONG L Y，CHEN Z W. Protective effects of total of flacone C on cerebral ischemia-reperfusion injury in rats［J］.Chin Pharmacol Bull(中国药理学通报)，2006， 22（11）：1383-1386. ［2］ LIU P B，CAI Y M，SONG J X. Effects of propofol on cell apoptosis in ischemia-reperfusion injury in rat brain ［J］. J Four Mil Med Univ（第四军医大学学报），2007，28（8）：682-684. ［3］ WANG W， SUN Y J， LUO C X， et al. nNOS specific inhibitor 7-nitrioindazole up-egulates the neurogenesis after focal cerebral ischemia in the adult mice［J］. Chin Pharmacol Bull(中国药理学通报)， 2006， 22(5)： 559 - 563. ［4］ KATO H， LIU Y， ARAKI T， et al.Tempeoral profile of the effects of pretreatment with brief cerebral ischemia on the neuron damage following secondary ischemia insult in the gerbil： cumulative damage and protective effects［J］.Brain Res， 1991， 553(2)：238-242. ［5］ HONG S R，YIN M H. Protection of extract from Ginkgo biloba leaves on cell apoptosis in mice induced by cerebral ischemia-reperfusion ［J］. Chin Tradit Herb Drugs（中草药）， 2007， 38(12)：1864-1867. ［6］ ZHANG D J， LI Y Z， ZHANG Z. Protective effect of ginseng total saponin on the neuroal injury in rat models with focal cerebral ischemic reperfusion ［J］. J Shandong Univ（Health Sci）（山东大学学报：医学版），2007，45（12）：1247-1252. ［7］ WANG P， WANG W， XU Y L， et al. Comparison of focal cerebral ischemia / reperfusion induced apoptosis of astrocytes and neurons in rats［J］. Chin J Histochem Cytochem (中国组织化学与细胞化学杂志)， 2006， 16(2)：113-118. ［8］ KALINICHENKO S G， MATVEEVA N I.Morphological characteristic of apoptosis and its significance in neurogenesis［J］.J Morfologiia， 2007， 131(2)：16-28. ［9］ PASCHENW， MENGESDORF T. Cellular abnormalities linked to endop lasmic reticulum dysfunction in cerebrovascular disease therapeutic potential［J］. Pharmacol Ther， 2005， 108 (3)： 362-375. ［10］ SZEGEZDI E， LOGUE S E， GORMAN A M， et al. Mediators of endop lasmic reticulum stress-induced apoptosis［J］. EMBO Rep， 2006， 7 (9)： 880-885. ［11］ LAMPL Y， BOAZ M， GILAD R， et al. Minocycline treatment in acute stroke： an open label， evaluator blinded study［J］. Neurology， 2007， 69 (14)： 1404-1410. ［12］ LIU Y， WONG T P， AARTS M， et al. NMDA Receptor subunits have differential roles in mediating excitotoxic neuronal death both in vitro and in vivo［J］ . J Neurosci， 2007， 27 (11)：2846-2857. ［13］ XU X H ，CHEN Y ，ZHENG X X. Effects of puerarin on neuronal apoptosis induced by cerebral ischemia in rats［J］. Chin Pharm J（中国药学杂志），2006，41（21）：1638-1631. ［14］ XU X H， ZHANG S M， YAN W M， et al. Development of cerebral infaction， apoptotic cell death and expression of X-chromosome-linked inhibitor of apoptosis protein following focal ischemia in rats［J］. Life Sci， 2006， 78(16)： 704-712. ［15］ MORIYAMA M， SATO T， INOUE H， et al. The neuropeptide neuromedin promotes inflammation by direct activation of mast cells［J］. JEM， 2005， 202(2)：217-224. ［16］ KALINICHENKO S G， MATVEEVA N I.Morphological characteristic of apoptosis and its significance in neurogenesis ［ J ］ . J Morfologiia， 2007， 131 (2)：16-28. ［17］ DOUGLAS R G， GUIDO K. Pharmacological manipulation of cell cleath： clinical applications in sight?［J］. J Clin Invest， 2005，115(6)：2610-2617. ［18］ ZHANG A L， ZHANG J W， SUN J L.The progress of EGB′s protective mechanism for cerebral ischemia［J］. China Pharm（中国药房）， 2005， 16 (8)：626-629. ［19］ ZHANG L， SU Z Q， CHEN L X . Mild hypothermia alleviates rat cerebral ischemia reperfusion injury by inhibiting apoptosis in two different pathways ［J］. J Apopl Nerv Dis（中风与神经疾病杂志）， 2006， 23 (3)：318-321. ［20］ CHEN X L， TANG H F， WANG S C . Effect of Leermai capsules on pallium neural apoptosis following late stage cerebral ischemia reper fusion injury in rats［J］. China Pharm(中国药房)，2008，19（27）：2089-2092.
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